通过相关纳米二次离子质谱（NanoSIMS）和电子显微镜对肺巨噬细胞摄取胺碘酮进行高分辨率亚细胞成像，作为药物诱导的磷脂沉积症的证据。
High-resolution sub-cellular imaging by correlative NanoSIMS and electron microscopy of amiodarone internalisation by lung macrophages as evidence for drug-induced phospholipidosis.
作者信息
Jiang Haibo, Passarelli Melissa K, Munro Peter M G, Kilburn Matt R, West Andrew, Dollery Colin T, Gilmore Ian S, Rakowska Paulina D
机构信息
Centre for Microscopy, Characterisation and Analysis, University of Western Australia, 35 Stirling Highway, Crawley, WA 6009, Australia.
National Centre of Excellence in Mass Spectrometry Imaging (NiCE-MSI), National Physical Laboratory, Hampton Road, Teddington, Middlesex TW11 0LW, UK.
出版信息
Chem Commun (Camb). 2017 Jan 26;53(9):1506-1509. doi: 10.1039/c6cc08549k.
Correlative NanoSIMS and EM imaging of amiodarone-treated macrophages shows the internalisation of the drug at a sub-cellular level and reveals its accumulation within the lysosomes, providing direct evidence for amiodarone-induced phospholipidosis. Chemical fixation using tannic acid effectively seals cellular membranes aiding intracellular retention of diffusible drugs.
胺碘酮处理巨噬细胞的相关纳米二次离子质谱（NanoSIMS）和电子显微镜（EM）成像显示该药物在亚细胞水平的内化，并揭示其在溶酶体内的积累，为胺碘酮诱导的磷脂沉积症提供了直接证据。使用单宁酸进行化学固定可有效封闭细胞膜，有助于细胞内保留可扩散药物。
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