通过简单生化试验快速检测胃幽门弯曲菌定植情况
Rapid detection of gastric Campylobacter pylori colonization by a simple biochemical test.
作者信息
Abdalla S, Marco F, Perez R M, Piqué J M, Bordas J M, Jimenez de Anta M T, Teres J
机构信息
Gastroenterology Department, Barcelona University, Spain.
出版信息
J Clin Microbiol. 1989 Nov;27(11):2604-5. doi: 10.1128/jcm.27.11.2604-2605.1989.
A simple and rapid urease test to detect Campylobacter pylori infection was evaluated with bacterial culture as the "gold standard." The test was compared with the Gram stain and the conventional Christensen urease test. The culture method detected C. pylori in 29 of 49 gastric biopsy specimens. The rapid urease test showed 27 positive samples within 1 h at 55 degrees C (specificity, 100%; sensitivity, 93%) and 18 at room temperature (specificity, 100%; sensitivity, 62%). The Gram stain exhibited a sensitivity of 86% and a specificity of 91%. The conventional Christensen urease test detected C. pylori in only 4, 10, and 18 samples after 24, 48, and 72 h, respectively (sensitivities, 12, 36, and 60%, respectively; specificities, 95, 95, and 83%, respectively). We conclude that the rapid urease test is simple and highly specific for the detection of C. pylori and that it can be performed with small amounts of sample.
以细菌培养作为“金标准”，对一种用于检测幽门螺杆菌感染的简单快速脲酶试验进行了评估。将该试验与革兰氏染色及传统的克里斯滕森脲酶试验进行了比较。培养方法在49份胃活检标本中的29份检测出幽门螺杆菌。快速脲酶试验在55摄氏度下1小时内显示27份阳性样本（特异性100%；敏感性93%），在室温下显示18份阳性样本（特异性100%；敏感性62%）。革兰氏染色的敏感性为86%，特异性为91%。传统的克里斯滕森脲酶试验在24、48和72小时后分别仅在4份、10份和18份样本中检测出幽门螺杆菌（敏感性分别为12%、36%和60%；特异性分别为95%、95%和83%）。我们得出结论，快速脲酶试验对于检测幽门螺杆菌简单且具有高度特异性，并且可以用少量样本进行检测。
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