Sanpool Oranuch, Laymanivong Sakhone, Thanchomnang Tongjit, Rodpai Rutchanee, Sadaow Lakkhana, Phosuk Issarapong, Maleewong Wanchai, Intapan Pewpan M
Department of Parasitology, Faculty of Medicine, Khon Kaen University, Khon Kaen, Thailand; Research and Diagnostic Center for Emerging Infectious Diseases, Mekong Health Science Institue, Khon Kaen University, Khon Kaen, Thailand; Faculty of Medicine, Mahasarakram University, Maha Sarakram, Thailand.
Research and Diagnostic Center for Emerging Infectious Diseases, Mekong Health Science Institue, Khon Kaen University, Khon Kaen, Thailand; Centre of Malariology, Parasitology and Entomology, Ministry of Health, Vientiane, Lao Democratic People's Republic.
Acta Trop. 2017 Apr;168:37-40. doi: 10.1016/j.actatropica.2017.01.006. Epub 2017 Jan 11.
Blastocystis sp. is the most common protist found in the intestinal tract of humans and animals. Blastocystis subtypes (STs) are classified based on the molecular phylogeny of the small subunit rRNA gene (SSU rDNA). At least 17 Blastocystis STs have been reported and, of these, STs 1-9 have been found in humans. This study revealed the presence of human Blastocystis STs in Lao People's Democratic Republic (Lao PDR). Fecal samples were collected from apparently healthy people from the central province of Khammouane and the southern province of Champasak. Fresh fecal samples, found to be positive for Blastocystis using microscopy, were individually cultured in Jones' medium and each sample was used for the amplification and sequencing of a fragment of SSU rDNA. BLAST searches and phylogenetic analysis confirmed the distribution of four Blastocystis STs: ST1 (64%), ST2 (8%), ST3 (24%) and ST7 (4%). This is the first report to provide molecular data revealing the prevalence of Blastocystis STs in apparently healthy people from Lao PDR.
芽囊原虫是在人类和动物肠道中发现的最常见的原生生物。芽囊原虫亚型（STs）是根据小亚基核糖体RNA基因（SSU rDNA）的分子系统发育进行分类的。已报道至少17种芽囊原虫STs，其中，STs 1-9在人类中被发现。本研究揭示了老挝人民民主共和国（老挝）存在人类芽囊原虫STs。从甘蒙省中部和占巴塞省南部表面健康的人群中采集粪便样本。使用显微镜检查发现芽囊原虫呈阳性的新鲜粪便样本，分别在琼斯培养基中培养，每个样本用于SSU rDNA片段的扩增和测序。BLAST搜索和系统发育分析证实了四种芽囊原虫STs的分布：ST1（64%）、ST2（8%）、ST3（24%）和ST7（4%）。这是第一份提供分子数据揭示老挝表面健康人群中芽囊原虫STs流行情况的报告。
