Hewson Chris, Capraro David, Burdach Jon, Whitaker Noel, Morris Kevin V
The University of New South Wales, Biotechnology and Biomedical Sciences, Sydney NSW 2052 Australia.
The University of New South Wales, Biotechnology and Biomedical Sciences, Sydney NSW 2052 Australia; Molecular and Experimental Medicine, The Scripps Research Institute, La Jolla, CA, United States, 92037; City of Hope - Beckman Research Institute, Center for Gene Therapy, Duarte, CA, 91010, United States.
Noncoding RNA Res. 2016 Oct;1(1):3-11. doi: 10.1016/j.ncrna.2016.06.001. Epub 2016 Jul 1.
Cells communicate with one another to create microenvironments and share resources. One avenue by which cells communicate is through the action of exosomes. Exosomes are extracellular vesicles that are released by one cell and taken up by neighbouring cells. But how exosomes instigate communication between cells has remained largely unknown. We present evidence here that particular long non-coding RNA molecules are preferentially packaged into exosomes. We also find that a specific class of these exosome associated non-coding RNAs functionally modulate cell viability by direct interactions with L-lactate dehydrogenase B (LDHB), high-mobility group protein 17 (HMG-17), and CSF2RB, proteins involved in metabolism, nucleosomal architecture and cell signalling respectively. Knowledge of this endogenous cell to cell pathway, those proteins interacting with exosome associated non-coding transcripts and their interacting domains, could lead to a better understanding of not only cell to cell interactions but also the development of exosome targeted approaches in patient specific cell-based therapies.
细胞相互通讯以创建微环境并共享资源。细胞通讯的一种途径是通过外泌体的作用。外泌体是由一个细胞释放并被邻近细胞摄取的细胞外囊泡。但是，外泌体如何促进细胞间通讯在很大程度上仍然未知。我们在此提供证据表明，特定的长链非编码RNA分子优先被包装到外泌体中。我们还发现，这类与外泌体相关的非编码RNA中的一类通过分别与参与代谢、核小体结构和细胞信号传导的蛋白质L-乳酸脱氢酶B（LDHB）、高迁移率族蛋白17（HMG-17）和CSF2RB直接相互作用，在功能上调节细胞活力。了解这种内源性细胞间途径、那些与外泌体相关的非编码转录本相互作用的蛋白质及其相互作用结构域，不仅可以更好地理解细胞间相互作用，还可以促进基于患者特异性细胞疗法的外泌体靶向方法的开发。
