Peter S Jerine, Basha S Kadar, Giridharan R, Lavinya B Udhaya, Sabina Evan Prince
School of Biosciences and Technology, VIT University, Vellore, India.
School of Biosciences and Technology, VIT University, Vellore, India.
Biomed Pharmacother. 2017 Apr;88:11-18. doi: 10.1016/j.biopha.2017.01.032. Epub 2017 Jan 13.
The non-steroidal anti-inflammatory drug (NSAID), diclofenac causes hepato-renal toxicity and gastric ulcer. The aim of this study was to investigate the protective effect of Spirulina fusiformis on Diclofenac-induced toxicity in Wistar albino rats.
Rats were treated as follows: normal control (group I); diclofenac (50mg/kgb.w., i.p.) treated rats (group II); diclofenac-induced (50mg/kgb.w., i.p.) rats treated with Spirulina fusiformis (400mg/kgb.w., p.o.) (group III); diclofenac-induced (50mg/kgb.w., i.p.) rats treated with silymarin (25mg/kgb.w., p.o.) (group IV); Spirulina fusiformis (400mg/kgb.w., p.o.) alone treated rats (groupV). Biochemical (liver and kidney functional markers) and antioxidant parameters (enzymic and non-enzymic antioxidants) were measured in the blood and tissue homogenates of the rats. Evaluation of intestinal ulcer score and assessment of liver and kidney histology were also done.
Alterations in the levels of biochemical and antioxidant assays and histopathological changes in liver and kidney proved the toxic effect of diclofenac. The ulcer score was significantly increased in the diclofenac treated rats. Spirulina fusiformis showed to reduce such changes and was able to restore normal antioxidant status in the rats.
Our study proves the hepato-renal and gastroprotective activity of Spirulina fusiformis in diclofenac-treated rats.
非甾体抗炎药双氯芬酸会导致肝肾毒性和胃溃疡。本研究的目的是调查螺旋藻对双氯芬酸诱导的Wistar白化大鼠毒性的保护作用。
大鼠按以下方式处理：正常对照组（第一组）；双氯芬酸（50mg/kg体重，腹腔注射）处理的大鼠（第二组）；双氯芬酸诱导（50mg/kg体重，腹腔注射）并用螺旋藻（400mg/kg体重，口服）处理的大鼠（第三组）；双氯芬酸诱导（50mg/kg体重，腹腔注射）并用水飞蓟宾（25mg/kg体重，口服）处理的大鼠（第四组）；单独用螺旋藻（400mg/kg体重，口服）处理的大鼠（第五组）。在大鼠的血液和组织匀浆中测量生化指标（肝脏和肾脏功能标志物）和抗氧化参数（酶促和非酶促抗氧化剂）。还进行了肠道溃疡评分评估以及肝脏和肾脏组织学评估。
生化和抗氧化测定水平的改变以及肝脏和肾脏的组织病理学变化证明了双氯芬酸的毒性作用。双氯芬酸处理的大鼠溃疡评分显著增加。螺旋藻显示可减轻此类变化，并能够恢复大鼠的正常抗氧化状态。
我们的研究证明了螺旋藻对双氯芬酸处理的大鼠具有肝肾保护和胃保护活性。
