颈部和胸部注射稀释羟基磷灰石后新胶原形成及皮肤力学性能改善的初步研究

Improved Neocollagenesis and Skin Mechanical Properties After Injection of Diluted Calcium Hydroxylapatite in the Neck and Décolletage:A Pilot Study.

作者信息

Yutskovskaya Yana Alexandrovna, Kogan Evgeniya Alexandrovna

出版信息

J Drugs Dermatol. 2017 Jan 1;16(1):68-74.

Abstract

BACKGROUND

Calcium hydroxylapatite (CaHA; Radiesse (R)) provides safe and effective correction of moderate-to-deep lines, volume replacement, lift and contour, and induction of neocollagenesis and neoelastogenesis for improved skin quality. CaHA hyperdilution takes advantage of its skin-tightening properties without a volumizing effect.

OBJECTIVE

To evaluate the collagen- and elastin-stimulating effects of diluted CaHA in subjects with skin laxity in the neck and décolletage.

METHODS

Twenty subjects with skin laxity in the neck and décolletage received multiple, linear, subdermal injections of CaHA diluted with preserved saline at baseline and 4 months: 1:2 dilution (normal skin), 1:4 dilution (thin skin), and 1:6 dilution (atrophic skin). Subjects also received deep subdermal injection of CaHA (~0.1 ml) of the same dilution in the peri-auricular area for skin biopsy. Biopsy tissue was obtained at baseline, 4 months, and 7 months for immunohistochemical evaluation of neocollagenesis. Changes to skin mechanical properties were measured by ultrasound scanning and cutometry. Subject and investigator satisfaction was evaluated using the Global Aesthetic Improvement Scale.

RESULTS

Immunohistochemical analysis of biopsy tissue demonstrated signi cant increases in collagen I expression at 4 months (P less than 0.05) and 7 months (P less than 0.00001) compared with baseline. Increases in collagen III levels were also significant at 4 months (P less than 0.00001); they declined by 7 months but remained above baseline. Staining for elastin and angiogenesis signi cantly increased at 4 months (P less than 0.05 and P less than 0.01, respectively) and 7 months (P less than 0.00001 for both) compared with baseline. Immunohistochemical data correlated with improvements in skin elasticity and pliability evaluated by cutometry, and with ultrasound-assessed increases in dermal thickness. Subject and investigator satisfaction was high, and the procedure was well tolerated.

CONCLUSIONS

Injection of diluted CaHA is very effective for skin tightening of the neck and décolletage. J Drugs Dermatol. 2017;16(1):68-74..

摘要

背景

羟基磷灰石钙（CaHA；瑞蓝唯瑅（R））可安全有效地改善中度至深度皱纹、补充容量、提升轮廓，并诱导新胶原蛋白和新弹性蛋白生成，从而改善皮肤质量。CaHA超稀释利用其紧致皮肤的特性，而无填充效果。

目的

评估稀释后的CaHA对颈部和胸部皮肤松弛受试者胶原蛋白和弹性蛋白的刺激作用。

方法

20名颈部和胸部皮肤松弛的受试者在基线期和4个月时接受多次皮下线性注射用含防腐剂生理盐水稀释的CaHA：1:2稀释度（正常皮肤）、1:4稀释度（薄皮肤）和1:6稀释度（萎缩皮肤）。受试者还在耳周区域接受相同稀释度的CaHA深部皮下注射（约0.1 ml）用于皮肤活检。在基线期、4个月和7个月时获取活检组织，用于新胶原蛋白生成的免疫组织化学评估。通过超声扫描和皮肤硬度测量仪测量皮肤力学性能的变化。使用全球美学改善量表评估受试者和研究者的满意度。

结果

活检组织的免疫组织化学分析显示，与基线相比，4个月时（P＜0.05）和7个月时（P＜0.00001）I型胶原蛋白表达显著增加。III型胶原蛋白水平在4个月时也显著增加（P＜0.00001）；7个月时有所下降，但仍高于基线。与基线相比，弹性蛋白和血管生成染色在4个月时（分别为P＜0.05和P＜0.01）和7个月时（两者均为P＜0.00001）显著增加。免疫组织化学数据与通过皮肤硬度测量仪评估的皮肤弹性和柔韧性改善以及超声评估的真皮厚度增加相关。受试者和研究者的满意度较高，且该操作耐受性良好。