Rammal Almoaidbellah, Tewfik Marc, Rousseau Simon
Department of Otolaryngology - Head and Neck Surgery, McGill University, Montreal, Canada.
Department of Otolaryngology - Head and Neck Surgery, King Abdul-Aziz University, Jeddah, Saudi Arabia.
J Otolaryngol Head Neck Surg. 2017 Jan 17;46(1):7. doi: 10.1186/s40463-016-0183-x.
Bacteria are suspected players in the pathogenesis of chronic rhinosinusitis (CRS), yet their exact role is not understood. We investigated the effect of planktonic and biofilm of staphylococcus aureus (SA) and Pseudomonas aeruginosa (PA) on the mucosa of CRS patients with gram-positive and gram-negative infections by measuring the levels of IL-6 and RANTES, a chemokine with activity on eosinophils and T lymphocytes.
Ethmoid mucosa of six CRS patients with gram-positive bacteria on culture and five with gram-negative bacteria were compared to ethmoid mucosa of 8 control patients. The tissue explants were stimulated with SA and PA extracts in planktonic and biofilm form for 6 hours, then RANTES levels were measured by ELISA.
Compared to the control group, CRS patients with gram-negative predominance demonstrated a significantly higher level of RANTES expression in response to all forms of bacterial stimuli (P-value <0.05). Patients with gram-positive predominance showed a higher level of RANTES compere to control group, however, this difference was not significant (P-value >0.05).
The mucosa of CRS patients with gram-negative infections has a heightened innate immune response compared to controls and patients with gram-positive infections. It is possible that this response leads to the pathological eosinophilic inflammation seen in CRS.
细菌被怀疑在慢性鼻窦炎（CRS）的发病机制中起作用，但其确切作用尚不清楚。我们通过测量白细胞介素-6（IL-6）和RANTES（一种对嗜酸性粒细胞和T淋巴细胞有活性的趋化因子）的水平，研究了金黄色葡萄球菌（SA）和铜绿假单胞菌（PA）的浮游菌和生物膜对革兰氏阳性和革兰氏阴性感染的CRS患者黏膜的影响。
将6例培养出革兰氏阳性菌的CRS患者和5例培养出革兰氏阴性菌的CRS患者的筛窦黏膜与8例对照患者的筛窦黏膜进行比较。用浮游菌和生物膜形式的SA和PA提取物刺激组织外植体6小时，然后通过酶联免疫吸附测定法（ELISA）测量RANTES水平。
与对照组相比，革兰氏阴性菌占优势的CRS患者在对所有形式的细菌刺激的反应中，RANTES表达水平显著更高（P值<0.05）。革兰氏阳性菌占优势的患者与对照组相比，RANTES水平更高，然而，这种差异不显著（P值>0.05）。
与对照组和革兰氏阳性菌感染的患者相比，革兰氏阴性菌感染的CRS患者的黏膜具有增强的固有免疫反应。这种反应可能导致CRS中出现的病理性嗜酸性粒细胞炎症。
