Oxburgh Leif, Carroll Thomas J, Cleaver Ondine, Gossett Daniel R, Hoshizaki Deborah K, Hubbell Jeffrey A, Humphreys Benjamin D, Jain Sanjay, Jensen Jan, Kaplan David L, Kesselman Carl, Ketchum Christian J, Little Melissa H, McMahon Andrew P, Shankland Stuart J, Spence Jason R, Valerius M Todd, Wertheim Jason A, Wessely Oliver, Zheng Ying, Drummond Iain A
Center for Molecular Medicine, Maine Medical Center Research Institute, Scarborough, Maine;
Department of Molecular Biology and.
J Am Soc Nephrol. 2017 May;28(5):1370-1378. doi: 10.1681/ASN.2016101077. Epub 2017 Jan 17.
(Re)Building a Kidney is a National Institute of Diabetes and Digestive and Kidney Diseases-led consortium to optimize approaches for the isolation, expansion, and differentiation of appropriate kidney cell types and the integration of these cells into complex structures that replicate human kidney function. The ultimate goals of the consortium are two-fold: to develop and implement strategies for engineering of replacement kidney tissue, and to devise strategies to stimulate regeneration of nephrons to restore failing kidney function. Projects within the consortium will answer fundamental questions regarding human gene expression in the developing kidney, essential signaling crosstalk between distinct cell types of the developing kidney, how to derive the many cell types of the kidney through directed differentiation of human pluripotent stem cells, which bioengineering or scaffolding strategies have the most potential for kidney tissue formation, and basic parameters of the regenerative response to injury. As these projects progress, the consortium will incorporate systematic investigations in physiologic function of and differentiated kidney tissue, strategies for engraftment in experimental animals, and development of therapeutic approaches to activate innate reparative responses.
“重建肾脏”是一个由美国国立糖尿病、消化和肾脏疾病研究所牵头的联盟，旨在优化从合适的肾细胞类型中进行分离、扩增和分化的方法，以及将这些细胞整合到复制人类肾脏功能的复杂结构中的方法。该联盟的最终目标有两个：一是制定并实施用于构建替代肾组织的策略，二是设计刺激肾单位再生以恢复衰竭肾功能的策略。联盟内的项目将解答有关发育中的肾脏中人类基因表达、发育中的肾脏不同细胞类型之间基本信号串扰、如何通过人类多能干细胞的定向分化获得肾脏的多种细胞类型、哪些生物工程或支架策略在肾脏组织形成方面最具潜力以及对损伤的再生反应基本参数等基本问题。随着这些项目的推进，该联盟将对分化后的肾脏组织的生理功能、在实验动物体内的植入策略以及激活先天性修复反应的治疗方法开展系统研究。
