Jain Pankaj G, Surana Sanjay J
R. C. Patel Institute of Pharmaceutical Education and Research, Shirpur, Dist-Dhule-425405, Maharashtra, India.
EXCLI J. 2016 Oct 25;15:599-613. doi: 10.17179/excli2016-394. eCollection 2016.
(L.) Druce (Leguminosae) (syn. L.) has antidiabetic and antioxidant potential. Earlier we reported its hypolipidemic activity obtained from ethanol extract (ET-PCF). Object of this work was to isolate ferulic acid (FA) from ET-PCF and evaluate hypolipidemic activity against high-fat diet (HFD)-induced hyperlipidemic laboratory rats. ET-PCF was subjected to flash column chromatography to isolate FA. The chemical structure of the isolated compound was elucidated by UV, IR, H NMR,C NMR and LC-MS. Further, the antihyperlipidemic effect of FA (10, 20 and 40 mg/kg, p.o.) in HFD-induced hyperlipidemic rats was investigated. Hyperlipidemia was induced in male Sprague-Dawley rats by feeding with HFD for 60 days. Lipid parameters such as total cholesterol (TC), Low-density lipoprotein cholesterol (LDL-C), high-density lipoprotein cholesterol (HDL-C) and triglycerides (TG) levels were measured in serum and hepatic tissue. Hepatic oxido-nitrosative stress (SOD, GSH, MDA and NO) were also determined. Histological evaluation of liver tissue was carried out. The structure of the isolated compound was characterized based on spectral data and confirmed as FA. HFD induced an alteration in serum, and hepatic lipid profile (triglyceride, cholesterol, HDL, and LDL) was significantly restored (p 0.001) by administration of FA (20 and 40 mg/kg, p.o.). The elevated level of oxido-nitrosative stress in liver was significantly reduced (p 0.001) by FA (20 and 40 mg/kg, p.o.). Histological aberration induced in the liver after HFD ingestion were restored by FA administration. Ferulic acid isolated from ET-PCF showed hypolipidemic effects in HFD-induced hyperlipidemic rats via modulation of elevated oxido-nitrosative stress.
（豆科）德鲁斯（L.）（豆科）具有抗糖尿病和抗氧化潜力。此前我们报道了从乙醇提取物（ET - PCF）中获得的其降血脂活性。这项工作的目的是从ET - PCF中分离出阿魏酸（FA），并评估其对高脂饮食（HFD）诱导的高脂血症实验大鼠的降血脂活性。对ET - PCF进行快速柱色谱法以分离FA。通过紫外光谱（UV）、红外光谱（IR）、氢核磁共振（H NMR）、碳核磁共振（C NMR）和液相色谱 - 质谱联用（LC - MS）对分离出的化合物的化学结构进行了阐明。此外，研究了FA（10、20和40毫克/千克，口服）对HFD诱导的高脂血症大鼠的抗高脂血症作用。通过给雄性斯普拉格 - 道利大鼠喂食HFD 60天来诱导高脂血症。测量血清和肝组织中的脂质参数，如总胆固醇（TC）、低密度脂蛋白胆固醇（LDL - C）、高密度脂蛋白胆固醇（HDL - C）和甘油三酯（TG）水平。还测定了肝脏氧化亚硝化应激（超氧化物歧化酶（SOD）、谷胱甘肽（GSH）、丙二醛（MDA）和一氧化氮（NO））。对肝组织进行了组织学评估。基于光谱数据对分离出的化合物的结构进行了表征，并确认为FA。HFD诱导血清变化，通过给予FA（20和40毫克/千克，口服），肝脂质谱（甘油三酯、胆固醇、HDL和LDL）得到显著恢复（p < 0.001）。FA（20和40毫克/千克，口服）显著降低了肝脏中升高的氧化亚硝化应激水平（p < 0.001）。给予FA可恢复HFD摄入后肝脏中诱导的组织学异常。从ET - PCF中分离出的阿魏酸通过调节升高的氧化亚硝化应激，对HFD诱导的高脂血症大鼠具有降血脂作用。
