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利用18S rRNA基因序列对印度西孟加拉邦养殖鲤鱼感染的**嗜子宫线虫属**和**嗜鱼虱属**(甲壳纲:锚头鱼虱科)进行分子特征分析。

Molecular characterization of and (Crustacea: Argulidae) infecting the cultured carps in West Bengal, India using 18S rRNA gene sequences.

作者信息

Patra Avijit, Mondal Anjan, Banerjee Sayani, Adikesavalu Harresh, Joardar Siddhartha Narayan, Abraham Thangapalam Jawahar

机构信息

Department of Aquatic Animal Health, Faculty of Fishery Sciences, West Bengal University of Animal and Fishery Sciences, Chakgaria, Kolkata, West Bengal, India.

Department of Veterinary Microbiology, Faculty of Veterinary and Animal Sciences, West Bengal University of Animal and Fishery Sciences, Belgachia, Kolkata, West Bengal, India.

出版信息

Mol Biol Res Commun. 2016 Sep;5(3):156-166.

PMID:28097169
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC5219910/
Abstract

The present study characterized spp. infecting the cultured carps using 18S rRNA gene sequences, estimated the genetic similarity among spp. and established their phylogenetic relationship. Of the 320 fish samples screened, 34 fish (10.6%) had infection. The parasitic frequency index (PFI) was observed to be high (20%) in and . The frequency of infection was high in September (PFI: 17%) and October (PFI: 12.9%). The 18S rRNA sequences of five (KF583878 KF192316, KM016968, KM016969, and KM016970) and one (KF583879) of this study showed genetic heterogeneity and exhibited 77-99% homology among the 18S rRNA gene sequences of spp. of NCBI GenBank database. Among the Indian spp. the sequence homology was 87-100%. Evolutionary pair-wise distances between Indian spp. and other spp. ranged from 0 to 20.20%. In the phylogenetic tree, all the crustaceans were clustered together as a separate clade with two distinct lineages. The lineage-1 comprised exclusive of Branchiura ( spp.). All clustered together and (KF583879) was closely related to sp. JN558648. The results of the present study provided baseline data for future work on population structure analysis of Indian species.

摘要

本研究利用18S rRNA基因序列对感染养殖鲤鱼的物种进行了特征分析,估计了这些物种之间的遗传相似性,并建立了它们的系统发育关系。在筛选的320份鱼类样本中,有34条鱼(10.6%)受到感染。在[具体物种1]和[具体物种2]中观察到寄生频率指数(PFI)较高(20%)。9月(PFI:17%)和10月(PFI:12.9%)的感染频率较高。本研究中五个[物种名称1](KF583878、KF192316、KM016968、KM016969和KM016970)和一个[物种名称2](KF583879)的18S rRNA序列显示出遗传异质性,在NCBI GenBank数据库的[物种名称1]物种的18S rRNA基因序列中同源性为77 - 99%。在印度的[物种名称1]物种中,序列同源性为87 - 100%。印度的[物种名称1]物种与其他[物种名称1]物种之间的进化成对距离在0到20.20%之间。在系统发育树中,所有甲壳类动物聚在一起形成一个单独的分支,有两个不同的谱系。谱系1仅包括鳃尾亚纲([物种名称3]物种)。所有[物种名称1]聚在一起,[物种名称2](KF583879)与[物种名称4] sp. JN558648密切相关。本研究结果为印度[物种名称1]物种的种群结构分析的未来工作提供了基线数据。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/7939/5219910/1975f82ca8bb/mbrc-5-156-g001.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/7939/5219910/1975f82ca8bb/mbrc-5-156-g001.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/7939/5219910/1975f82ca8bb/mbrc-5-156-g001.jpg

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