Soni Kapil, Parle Milind
Department of Pharmaceutical Sciences, Guru Jambheshwar University of Science and Technology, Hisar, 125001, Haryana, India.
Neurochem Res. 2017 May;42(5):1333-1344. doi: 10.1007/s11064-017-2177-0. Epub 2017 Jan 17.
The present study was designed to explore the beneficial effects of successive 10 days administration of Trachyspermum ammi seed's powder (TASP) along with diet (at the dose of 0.5%, 1.0% and 2.0% w/w) on learning and memory of mice. A total of 306 mice divided in 51 equal groups were employed in the study. Passive avoidance paradigm (PAP) and Object recognition Task (ORT) were employed as exteroceptive models. The brain acetylcholinesterase activity (AChE), serum cholesterol, brain monoaldehyde (MDA), brain reduced glutathione (GSH) and brain nitrite were estimated and Alprazolam, Scopolamine and Electroshock induced amnesia was employed to describe the actions. Treatment of TASP significantly increased step down latency of PAA and significantly increased discrimination index of ORT in groups with or without amnesia when compared to respective control groups. Furthermore, TASP administration resulted in significant fall in brain AChE activity, brain MDA level and brain nitrite level with simultaneous rise in brain GSH level, thereby decreased oxidative damage. A significant decrease in serum cholesterol was also observed. Ajowan supplementation may prove a remedy for the management of cognitive disorders owing to have pro-cholinergic, antioxidant and hypo-lipidemic activities.
本研究旨在探讨连续10天给予小茴香籽粉（TASP）并添加到饮食中（剂量为0.5%、1.0%和2.0% w/w）对小鼠学习和记忆的有益影响。本研究共使用了306只小鼠，分为51个相等的组。采用被动回避范式（PAP）和物体识别任务（ORT）作为外感受性模型。测定了脑乙酰胆碱酯酶活性（AChE）、血清胆固醇、脑丙二醛（MDA）、脑还原型谷胱甘肽（GSH）和脑亚硝酸盐，并采用阿普唑仑、东莨菪碱和电击诱导的失忆来描述其作用。与各自的对照组相比，TASP治疗显著增加了PAA的步下潜伏期，并显著增加了有或无失忆组ORT的辨别指数。此外，TASP给药导致脑AChE活性、脑MDA水平和脑亚硝酸盐水平显著下降，同时脑GSH水平升高，从而减少了氧化损伤。血清胆固醇也显著降低。由于小茴香具有促胆碱能、抗氧化和降血脂活性，补充小茴香可能是治疗认知障碍的一种方法。
