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Analyst. 2017 Apr 10;142(8):1381-1386. doi: 10.1039/c6an01916a.
Mid-infrared microscopy has become a key technique in the field of biomedical science and spectroscopy. This label-free, non-destructive technique permits the visualisation of a wide range of intrinsic biochemical markers in tissues, cells and biofluids by detection of the vibrational modes of the constituent molecules. Together, infrared microscopy and chemometrics is a widely accepted method that can distinguish healthy and diseased states with high accuracy. However, despite the exponential growth of the field and its research world-wide, several barriers currently exist for its full translation into the clinical sphere, namely sample throughput and data management. The advent and incorporation of quantum cascade lasers (QCLs) into infrared microscopes could help propel the field over these remaining hurdles. Such systems offer several advantages over their FT-IR counterparts, a simpler instrument architecture, improved photon flux, use of room temperature camera systems, and the flexibility of a tunable illumination source. In this current study we explore the use of a QCL infrared microscope to produce high definition, high throughput chemical images useful for the screening of biopsied colorectal tissue.
中红外显微镜已成为生物医学科学和光谱学领域的关键技术。这种无标记、非破坏性的技术可以通过检测组成分子的振动模式来可视化组织、细胞和生物流体中广泛的固有生化标志物。红外显微镜和化学计量学的结合是一种被广泛接受的方法，可以高精度地区分健康和患病状态。然而，尽管该领域在全球范围内呈指数级增长，但仍存在一些障碍限制了其在临床领域的全面转化，即样品通量和数据管理。量子级联激光器（QCL）的出现和纳入红外显微镜可能有助于克服这些剩余的障碍。与傅里叶变换红外（FT-IR）相比，这些系统具有几个优势，包括更简单的仪器结构、更高的光子通量、使用室温相机系统以及可调谐照明源的灵活性。在本研究中，我们探索了使用 QCL 红外显微镜生成高清晰度、高通量化学图像的方法，这些图像可用于筛查活检的结直肠组织。
