Sirisena Sameera, Zabaras Dimitrios, Ng Ken, Ajlouni Said
Faculty of Veterinary and Agricultural Sciences, Univ. of Melbourne, Melbourne, VIC, 3010, Australia.
CSIRO Food and Nutrition, North Ryde, NSW, 2113, Australia.
J Food Sci. 2017 Feb;82(2):333-340. doi: 10.1111/1750-3841.13625. Epub 2017 Jan 18.
Date (Pheonix dactylifera L.) seeds are a valuable and abundant by-product with various potential food applications. Free polyphenols (FPPs) and bound polyphenols (BPPs) of date seeds from Deglet Nour variety grown in Australia were investigated using high-performance liquid chromatography-mass spectrometry. The FPP fraction contained the following main phenolic compounds per gram of date seed powder; procyanidin B1 (499.8 ± 7.8 μg), procyanidin B2 (288.6 ± 6.1 μg), catechin (167.6 ± 2.1 μg), epicatechin (39.44 ± 0.39 μg), and protocatechuic acid (1.77 ± 0.22 μg). Additionally, one of the 2 A-type dimers was confirmed as procyanidin A2 (24.05 ± 0.12 μg/g). A-type dimers have not been reported before in date seeds. The BPP fraction contained epicatechin (52.59 ± 0.76 μg/g) and procyanidin B2 (294.2 ± 3.7 μg/g), while several peaks exhibiting ESI m/z of 153 indicated dihydroxybenzoic acid isomers including protocatechuic acid (2.138 ± 0.025 μg/g). These findings contributed to our knowledge of date seed phytochemicals and understanding of their contribution to the reported bioactivities.
海枣（Phoenix dactylifera L.）种子是一种具有多种潜在食品应用价值且产量丰富的副产品。采用高效液相色谱 - 质谱联用技术对在澳大利亚种植的Deglet Nour品种海枣种子中的游离多酚（FPPs）和结合多酚（BPPs）进行了研究。每克海枣种子粉末的FPP部分含有以下主要酚类化合物：原花青素B1（499.8±7.8μg）、原花青素B2（288.6±6.1μg）、儿茶素（167.6±2.1μg）、表儿茶素（39.44±0.39μg）和原儿茶酸（1.77±0.22μg）。此外，确认其中一种2种A型二聚体为原花青素A2（24.05±0.12μg/g）。此前尚未有关于海枣种子中A型二聚体的报道。BPP部分含有表儿茶素（52.59±0.76μg/g）和原花青素B2（294.2±3.7μg/g），而几个显示电喷雾电离质荷比为153的峰表明存在二羟基苯甲酸异构体，包括原儿茶酸（2.138±0.025μg/g）。这些发现有助于我们了解海枣种子的植物化学物质，并理解它们对所报道生物活性的贡献。
