Shakoor Hira, Abdelfattah Fatima, Albadi Khaula, Adib Mentalla, Kizhakkayil Jaleel, Platat Carine
Department of Food, Nutrition and Health, College of Food and Agriculture, United Arab Emirates University, P.O. Box 15551, Al Ain, UAE.
Evid Based Complement Alternat Med. 2020 Jul 29;2020:4290702. doi: 10.1155/2020/4290702. eCollection 2020.
Type 2 diabetes mellitus is increasing worldwide, and the United Arab Emirates is presenting one of the world's highest prevalence rates. Dietary polyphenols exert an antidiabetic effect by modulating carbohydrates digestion and cellular glucose uptake. Due to their particularly high content in polyphenols, date seeds represent a potential antidiabetic agent. This study aims to determine if date seed polyphenols inhibit the activity of the enzymes (-amylase and -glucosidase), responsible for the digestion of carbohydrates and modulating the glucose uptake by human liver cells. activity of the intestinal -glucosidase, pancreatic -amylase, the glucose uptake by HepG2 cells, and the expression of GLUT4 and AMPK analyzed by western blotting (with and without date seeds extract). Our result showed that the maximum enzymes inhibition was obtained with 400 g/mL and 900 g/mL DSE for -amylase and -glucosidase, respectively. The HepG2 cell viability significantly decreased up to 80% at 4000 g/mL DSE. The expression of GLUT4 was higher at 100 g/mL DSE (with insulin and without insulin). However, the expressions of P-AMPK and AMPK were increased by DSE, mainly in a non-insulin-dependent manner. Therefore, DSE, by inhibiting carbohydrate digestion and stimulating glucose uptake by HepG2, can potentially demonstrate the therapeutic potential for diabetes management.
2型糖尿病在全球范围内呈上升趋势，阿拉伯联合酋长国是世界上患病率最高的国家之一。膳食多酚通过调节碳水化合物消化和细胞葡萄糖摄取发挥抗糖尿病作用。由于枣籽中多酚含量特别高，因此枣籽是一种潜在的抗糖尿病药物。本研究旨在确定枣籽多酚是否能抑制负责碳水化合物消化和调节人肝细胞葡萄糖摄取的酶（α-淀粉酶和α-葡萄糖苷酶）的活性。通过蛋白质印迹法分析肠道α-葡萄糖苷酶、胰腺α-淀粉酶的活性、HepG2细胞对葡萄糖的摄取以及GLUT4和AMPK的表达（有无枣籽提取物）。我们的结果表明，对于α-淀粉酶和α-葡萄糖苷酶，分别在400μg/mL和900μg/mL的枣籽提取物（DSE）下可获得最大酶抑制率。在4000μg/mL的DSE下，HepG2细胞活力显著下降高达80%。在100μg/mL的DSE下（有胰岛素和无胰岛素），GLUT4的表达更高。然而，DSE可增加P-AMPK和AMPK的表达，主要以非胰岛素依赖的方式。因此，DSE通过抑制碳水化合物消化和刺激HepG2细胞摄取葡萄糖，可能具有治疗糖尿病的潜在潜力。
