桑科（Moraceae）EST-SSR标记的开发与特征分析
Development and characterization of EST-SSR markers for (Moraceae).
作者信息
Liu Haijun, Tan Weizheng, Sun Hongbin, Liu Yu, Meng Kaikai, Liao Wenbo
机构信息
Rescue Center of Wildlife in Shenzhen, Shenzhen 518040, People's Republic of China.
State Key Laboratory of Biocontrol and Guangdong Key Laboratory of Plant Resources, Sun Yat-sen University, Guangzhou 510275, People's Republic of China.
出版信息
Appl Plant Sci. 2016 Dec 9;4(12). doi: 10.3732/apps.1600113. eCollection 2016 Dec.
PREMISE OF THE STUDY
Polymorphic microsatellite markers were developed for (Moraceae), a threatened species endemic to China, to investigate the genetic diversity and structure of the species.
METHODS AND RESULTS
Based on the transcriptome data of , 63 primer pairs were preliminarily designed and tested, of which 34 were successfully amplified and 10 displayed clear polymorphisms across the 67 individuals from four populations of . The results showed the number of alleles per locus ranged from three to 10, and the observed heterozygosity and expected heterozygosity per locus varied from 0.000 to 0.706 and from 0.328 to 0.807, respectively.
CONCLUSIONS
These microsatellite markers will be useful in exploring genetic diversity and structure of . Furthermore, most loci were successfully cross-amplified in and , indicating that they will be of great value for genetic study across this genus.
研究目的
为中国特有的濒危物种构树（桑科）开发多态性微卫星标记，以研究该物种的遗传多样性和结构。
方法与结果
基于构树的转录组数据，初步设计并测试了63对引物，其中34对成功扩增，10对在来自构树四个种群的67个个体中显示出明显的多态性。结果表明，每个位点的等位基因数在3到10之间，每个位点的观察杂合度和期望杂合度分别在0.000到0.706和0.328到0.807之间。
结论
这些微卫星标记将有助于探索构树的遗传多样性和结构。此外，大多数位点在柘树和天仙果中成功进行了跨物种扩增，表明它们对该属的遗传研究具有重要价值。
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