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2013 - 2016年中国新疆野生动物和家畜小反刍兽疫的诊断

Diagnosis of Peste des Petits Ruminants in Wild and Domestic Animals in Xinjiang, China, 2013-2016.

作者信息

Li J, Li L, Wu X, Liu F, Zou Y, Wang Q, Liu C, Bao J, Wang W, Ma W, Lin H, Huang J, Zheng X, Wang Z

机构信息

OIE Reference Laboratory for Peste des Petits Ruminants, National Research Center for Exotic Animal Diseases, China Animal Health and Epidemiology Center, Qingdao, Shandong, China.

Xinjiang Uyghur Autonomous Region Animal Health Supervision Center, Urumqi, Xinjiang, China.

出版信息

Transbound Emerg Dis. 2017 Dec;64(6):e43-e47. doi: 10.1111/tbed.12600. Epub 2017 Jan 18.

Abstract

Peste des petits ruminants viruses (PPRVs) re-emerged in China at the end of 2013 and then spread rapidly into 22 provinces through movement of live goats and sheep. In this study, 96 samples of domestic animals and 13 samples of wildlife were analysed for the presence of PPRV infection by ELISA or RT-PCR. Of 96 samples from sheep and goats, 91 were PPRV positive, whereas all of the 13 samples from three wild species, Capra ibex (Capra ibex sibirica), argali (Ovis ammon) and Goitered gazelle (Gazella subgutturosa), were found to be positive. Five wildlife-origin isolates from the above samples were identified as the lineage IV by a multiple alignment of the partial sequences in N gene.

摘要

小反刍兽疫病毒(PPRVs)于2013年底在中国再次出现,随后通过活羊和山羊的移动迅速传播到22个省份。在本研究中,通过酶联免疫吸附测定(ELISA)或逆转录聚合酶链反应(RT-PCR)分析了96份家畜样本和13份野生动物样本中PPRV感染情况。在96份绵羊和山羊样本中,91份为PPRV阳性,而来自三种野生动物,即西伯利亚北山羊(Capra ibex sibirica)、盘羊(Ovis ammon)和鹅喉羚(Gazella subgutturosa)的所有13份样本均被检测为阳性。通过对上述样本中N基因部分序列的多重比对,从其中5份野生动物来源的分离株鉴定为IV系。

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