Shergy W J, Kredich D W, Pisetsky D S
Department of Medicine, VA Medical Center, Durham, NC.
J Rheumatol. 1989 Oct;16(10):1329-34.
To characterize patterns of autoantibody expression in pediatric and adult systemic lupus erythematosus (SLE), IgG and IgM antibodies to cardiolipin (aCL) and single-stranded DNA (anti-DNA) were measured in sera from 32 pediatric and 36 adult patients. Antibody levels were assessed by isotype-specific ELISA and compared with 15 pediatric disease controls and 36 healthy adult controls. These determinations revealed significant differences in the pattern of IgG and IgM autoantibody expression in adult and pediatric patients. IgM aCL were more common in the pediatric than adult SLE population with 11 of 32 pediatric patients being positive compared to only 4 of 36 in the adult group (p less than 0.05). Conversely, enhanced IgG autoantibody production was observed in the adult group as 27 of 36 adult patients were positive for IgG anti-DNA compared to only 16 of 32 children (p less than 0.05). Analysis of sequential sera showed differences in the temporal expression of IgG and IgM autoantibodies as well as variability between anti-DNA and aCL responses over time. Our data suggest differences in the isotype profile of autoantibody responses in pediatric and adult SLE and provide further evidence for heterogeneity in the mechanisms of autoantibody production.
为了描述儿童和成人系统性红斑狼疮（SLE）中自身抗体表达模式，检测了32例儿童和36例成人患者血清中抗心磷脂（aCL）和单链DNA（抗DNA）的IgG和IgM抗体。通过同型特异性酶联免疫吸附测定（ELISA）评估抗体水平，并与15例儿童疾病对照和36例健康成人对照进行比较。这些测定揭示了成人和儿童患者中IgG和IgM自身抗体表达模式的显著差异。IgM aCL在儿童SLE患者中比成人更常见，32例儿童患者中有11例呈阳性，而成人组36例中只有4例阳性（p<0.05）。相反，在成人组中观察到IgG自身抗体产生增强，36例成人患者中有27例IgG抗DNA呈阳性，而32例儿童中只有16例阳性（p<0.05）。对连续血清的分析显示IgG和IgM自身抗体的时间表达存在差异，以及抗DNA和aCL反应随时间的变异性。我们的数据表明儿童和成人SLE自身抗体反应的同型谱存在差异，并为自身抗体产生机制的异质性提供了进一步证据。
