Li Jiuxuan, Wang Ying, Jin Huifang, Li Wujiao, Yan Chaochao, Yan Pengfei, Zhang Xiuyue, He Shunping, Song Zhaobin
Sichuan Key Laboratory of Conservation Biology on Endangered Wildlife, College of Life Sciences, Sichuan University, Chengdu 610065, PR China.
Sichuan Key Laboratory of Conservation Biology on Endangered Wildlife, College of Life Sciences, Sichuan University, Chengdu 610065, PR China; Key Laboratory of Aquatic Biodiversity and Conservation of Chinese Academy of Sciences, Institute of Hydrobiology, Chinese Academy of Sciences, Wuhan 430072, PR China; School of Life Sciences, Jianghan University, Wuhan 430056, PR China.
Gene. 2017 Mar 20;605:12-19. doi: 10.1016/j.gene.2016.11.045. Epub 2016 Dec 28.
The genus Triplophysa is the largest and most difficult to identity morphologically fish group of superfamily Cobitoidea with 140 currently valid species, and is mainly distributed in the Qinghai-Tibetan Plateau (QTP) and adjacent regions. Most species within this genus possess highly similar morphological characteristics for adaption to the highland environment and are very difficult to be identified only based on morphology. The traditional species identification, mainly based on external morphological diagnostic characters, leads to inconsistent results in many cases. Herein, we provided a molecular method based on mitochondrial cytochrome c subunit I (COI) for the identification of Triplophysa fishes. Thirty-three Triplophysa species, 244 individuals, were used to determine whether barcoding was effective in discriminating species for this genus. The mean intraspecific and interspecific K2P distances ranged from 0 to 14.9% (mean, 2.9%) and 0 to 23.4% (mean, 9.7%), respectively. The tree-based analysis displayed most of species formed discrete clusters with strong bootstrap support values (>90%). The results showed that most of Triplophysa species could be identified by DNA barcode and indicated DNA barcode could be used as a molecular marker for these species.
高原鳅属是鳅超科中最大且形态学上最难鉴定的鱼类类群，目前有140个有效物种，主要分布于青藏高原及其邻近地区。该属内的大多数物种具有高度相似的形态特征以适应高原环境，仅基于形态很难进行鉴定。传统的物种鉴定主要基于外部形态诊断特征，在很多情况下会导致结果不一致。在此，我们提供了一种基于线粒体细胞色素c亚基I（COI）的分子方法来鉴定高原鳅属鱼类。使用33种高原鳅属物种、244个个体来确定条形码技术在区分该属物种方面是否有效。种内和种间的平均K2P距离分别在0至14.9%（平均为2.9%）和0至23.4%（平均为9.7%）之间。基于树的分析显示，大多数物种形成了具有强自展支持值（>90%）的离散聚类。结果表明，大多数高原鳅属物种可以通过DNA条形码鉴定，这表明DNA条形码可作为这些物种的分子标记。
