Matsuo Yuichi, Kido Yasutoshi, Akada Junko, Shiota Seiji, Binh Tran Thanh, Trang Tran Thi Huyen, Dung Ho D Q, Tung Pham Huu, Tri Tran Dinh, Thuan Ngo P Minh, Tam Le Quang, Nam Bui Chi, Khien Vu Van, Yamaoka Yoshio
Yuichi Matsuo, Yasutoshi Kido, Junko Akada, Seiji Shiota, Tran Thanh Binh, Tran Thi Huyen Trang, Yoshio Yamaoka, Department of Environmental and Preventive Medicine, Oita University Faculty of Medicine, Yufu, Oita 879-5593, Japan.
World J Gastroenterol. 2017 Jan 7;23(1):48-59. doi: 10.3748/wjg.v23.i1.48.
To develop a novel () CagA antibody enzyme-linked immunosorbent assay (ELISA) suitable for detecting serum anti-CagA antibodies with high sensitivity.
Recombinant East Asian-type CagA protein was purified and immobilized for ELISA. Serum samples from 217 Vietnamese individuals (110 -infected and 107 uninfected individuals) were applied. Conventional ELISA from Western-type CagA and our East Asian-type CagA ELISA were evaluated by comparing 38 subjects with the Western-type genotype and 72 subjects with the East Asian-type genotype. Histological scores of the gastric mucosa were determined using the updated Sydney System to examine the relationship with anti-CagA antibody titers.
Recombinant 70-100 kDa fragments were immobilized on the ELISA plate. In ROC analysis, the area under the curve of our East Asian-type CagA ELISA was comparable to that of conventional CagA ELISA. The sensitivity of the two ELISAs differed depending on the genotype. The sensitivity of East Asian-type CagA ELISA was higher for subjects infected with East Asian-type ( < 0.001), and the sensitivity of the conventional CagA ELISA tended to be higher for subjects infected with Western ( = 0.056). The titer of anti-CagA antibody tended to correlate with monocyte infiltration scores ( = 0.25, = 0.058) and was inversely correlated with density ( = -0.26, = 0.043).
The novel ELISA is useful to detect anti-CagA antibodies in East Asian countries, and the titer may be a marker for predicting chronic gastritis.
开发一种新型的细胞毒素相关基因A（CagA）抗体酶联免疫吸附测定（ELISA），用于高灵敏度检测血清抗CagA抗体。
纯化重组东亚型CagA蛋白并固定用于ELISA。应用来自217名越南个体（110名感染个体和107名未感染个体）的血清样本。通过比较38名具有西方型基因型的受试者和72名具有东亚型基因型的受试者，评估来自西方型CagA的传统ELISA和我们的东亚型CagA ELISA。使用更新的悉尼系统确定胃黏膜的组织学评分，以检查与抗CagA抗体滴度的关系。
重组70 - 100 kDa片段固定在ELISA板上。在ROC分析中，我们的东亚型CagA ELISA的曲线下面积与传统CagA ELISA相当。两种ELISA的灵敏度因基因型而异。东亚型CagA ELISA对感染东亚型幽门螺杆菌的受试者灵敏度更高（P < 0.001），而传统CagA ELISA对感染西方型幽门螺杆菌的受试者灵敏度往往更高（P = 0.056）。抗CagA抗体滴度与单核细胞浸润评分呈正相关趋势（r = 0.25，P = 0.058），与幽门螺杆菌密度呈负相关（r = -0.26，P = 0.043）。
这种新型ELISA对于检测东亚国家的抗CagA抗体很有用，并且该滴度可能是预测慢性胃炎的一个标志物。
