艰难梭菌R20291ΔPaLoc模型菌株的开发及体外方法表明，细胞致死性膨胀毒素（CDT）产生细胞毒性水平需要CdtR。
Development of Clostridium difficile R20291ΔPaLoc model strains and in vitro methodologies reveals CdtR is required for the production of CDT to cytotoxic levels.
作者信息
Bilverstone T W, Kinsmore N L, Minton N P, Kuehne S A
机构信息
Clostridia Research Group, BBSRC/EPSRC Synthetic Biology Research Centre (SBRC), School of Life Sciences, Centre for Biomolecular Sciences, The University of Nottingham, Nottingham, NG7 2RD, UK.
Clostridia Research Group, BBSRC/EPSRC Synthetic Biology Research Centre (SBRC), School of Life Sciences, Centre for Biomolecular Sciences, The University of Nottingham, Nottingham, NG7 2RD, UK; NIHR Nottingham Digestive Diseases, (NDCC) Biomedical Research Unit, Nottingham University Hospitals NHS Trust and the University of Nottingham, NG7 2RD, UK.
出版信息
Anaerobe. 2017 Apr;44:51-54. doi: 10.1016/j.anaerobe.2017.01.009. Epub 2017 Jan 17.
Assessing the regulation of Clostridium difficile transferase (CDT), is complicated by the presence of a Pathogenicity locus (PaLoc) which encodes Toxins A and B. Here we developed R20291ΔPaLoc model strains and cell-based assays to quantify CDT-mediated virulence. Their application demonstrated that the transcriptional regulator, CdtR, was required for CDT-mediated cytotoxicity.
由于存在编码毒素A和毒素B的致病位点（PaLoc），评估艰难梭菌转移酶（CDT）的调控变得复杂。在此，我们构建了R20291ΔPaLoc模型菌株和基于细胞的检测方法来量化CDT介导的毒力。它们的应用表明，转录调节因子CdtR是CDT介导的细胞毒性所必需的。
Zotero 插件