TechLab Inc., Blacksburg, VA 24060-6358, USA.
Anaerobe. 2011 Aug;17(4):161-5. doi: 10.1016/j.anaerobe.2011.02.005. Epub 2011 Mar 3.
Clostridium difficile is a major enteropathogen of humans. It produces two main virulence factors, toxins A and B. A third, less well known toxin, C. difficile toxin (CDT), is a binary toxin composed of distinct enzymatic (CdtA) and cell binding/translocation (CdtB) proteins. We used a novel enzyme linked immunoassay (EIA) to detect CdtB protein in feces and culture fluids. Additionally, PCR was used to assay C. difficile isolates from fecal samples for the CDT locus (CdtLoc). Although the results from 80 isolates suggest no relationship between toxin concentrations in situ and in vitro, there is a good correlation between PCR detection of the cdtB gene and EIA detection of CdtB protein in vitro. Possible implications of the detection of CDT in patients are discussed.
艰难梭菌是人类的主要肠道病原体。它产生两种主要的毒力因子，毒素 A 和 B。第三种，不太知名的毒素，艰难梭菌毒素（CDT），是一种由独特的酶（CdtA）和细胞结合/易位（CdtB）蛋白组成的二元毒素。我们使用一种新的酶联免疫吸附试验（EIA）来检测粪便和培养液中的 CdtB 蛋白。此外，PCR 用于检测粪便样本中的艰难梭菌分离株的 CDT 基因座（CdtLoc）。虽然 80 个分离株的结果表明原位和体外毒素浓度之间没有关系，但 cdtB 基因的 PCR 检测与体外 CdtB 蛋白的 EIA 检测之间存在良好的相关性。讨论了在患者中检测 CDT 的可能意义。
