Ding Jiun-Yan, Chen Sheng-Chung, Lai Mei-Chin, Liao Tsai-Ling
Department of Life Sciences, National Chung Hsing University, Taichung, Taiwan, ROC.
Agricultural Biotechnology Center, National Chung Hsing University, Taichung, Taiwan, ROC.
Int J Syst Evol Microbiol. 2017 May;67(5):1333-1338. doi: 10.1099/ijsem.0.001811. Epub 2017 May 30.
A pleomorphic, gas-vesicle-containing, halophilic archaeon, designated strain H13T, was isolated from the solar saltern of the Western Salt Co., Chula Vista, California, USA. Cells of strain H13T were non-motile, rod-shaped and 3-10 µm in length. The optimum growth conditions were 3.5-5.0 M NaCl, 45-55 °C, and pH range of 6.5-8.2. The major polar lipids were C20C20 and C20C25 derivatives of phosphatidylglycerol, phosphatidylglycerol phosphate methyl ester and disulfated diglycosyl diether-1. The G+C content of he genome of strain H13T was calculated as 65.10 mol%. Phylogenetic analysis of 16S rRNA and rpoB' genes revealed that strain H13 was most closely related to Haloterrigena saccharevitans AB14T (16S rRNA gene sequence similarity: 99.51 %; rpoB' sequence similarity: 96.19 %) and Haloterrigena thermotolerans PR5T (99.11 %; 95.50 %). Strain H13T showed low genome relatedness values with Htg. saccharevitans AB14T and Htg. thermotolerans PR5T based on estimated average nucleotide identity (ANI; 92.59 and 91.68 %, respectively) and genome-to-genome distance analysis (GGDA; 47.90 and 45.00 %, respectively). Based on the phenotypic, chemotaxonomic and phylogenetic properties and the genome relatedness, it is evident that strain H13T represents a novel species of the genus Haloterrigena, for which the name Haloterrigena mahiisp. nov. is proposed. The type strain is H13T (=BCRC 910151T=NBRC 111885T).
从美国加利福尼亚州丘拉维斯塔市西部盐业公司的太阳能盐场中分离出了一株多形态、含气荚膜、嗜盐古菌，命名为菌株H13T。菌株H13T的细胞无运动性，呈杆状，长度为3-10 µm。最佳生长条件为3.5-5.0 M NaCl、45-55 °C，pH范围为6.5-8.2。主要极性脂类为磷脂酰甘油、磷脂酰甘油磷酸甲酯和双硫酸化二糖基二醚-1的C20C20和C20C25衍生物。菌株H13T基因组的G+C含量经计算为65.10 mol%。对16S rRNA和rpoB'基因的系统发育分析表明，菌株H13与嗜盐地芽孢杆菌AB14T（16S rRNA基因序列相似性：99.51%；rpoB'序列相似性：96.19%）和嗜热嗜盐地芽孢杆菌PR5T（99.11%；95.50%）关系最为密切。基于估计的平均核苷酸同一性（ANI；分别为92.59%和91.68%）和全基因组距离分析（GGDA；分别为47.90%和45.00%），菌株H13T与嗜盐地芽孢杆菌AB14T和嗜热嗜盐地芽孢杆菌PR5T的基因组相关性值较低。基于表型、化学分类和系统发育特性以及基因组相关性，很明显菌株H13T代表嗜盐地芽孢杆菌属的一个新物种，为此提出了新名称嗜盐地芽孢杆菌mahiisp. nov.。模式菌株为H13T（=BCRC 910151T=NBRC 111885T）。
