State Key Laboratory of Microbial Technology, Shandong University, 250100, China.
State Key Laboratory of Microbial Technology, Shandong University, 250100, China; National Glycoengineering Research Center, Shandong University, 250100, China.
Bioresour Technol. 2017 Apr;229:88-95. doi: 10.1016/j.biortech.2017.01.011. Epub 2017 Jan 12.
Cellulose conversion decreases significantly with increasing solid concentrations during enzymatic hydrolysis of insoluble lignocellulosic materials. Here, mass transfer limitation was identified as a significant determining factor of this decrease by studying the hydrolysis of delignified corncob residue in shake flask, the most used reaction vessel in bench scale. Two mass transfer efficiency-related factors, mixing speed and flask filling, were shown to correlate closely with cellulose conversion at solid loadings higher than 15% DM. The role of substrate characteristics in mass transfer performance was also significant, which was revealed by the saccharification of two corn stover substrates with different pretreatment methods at the same solid loading. Several approaches including premix, fed-batch operation, and particularly the use of horizontal rotating reactor were shown to be valid in facilitating cellulose conversion via improving mass transfer efficiency at solid concentrations higher than 15% DM.
在不溶性木质纤维素材料的酶水解过程中，随着固含量的增加，纤维素的转化率显著降低。通过在摇瓶中对脱木质素玉米芯残渣进行水解研究（这是在实验室规模中最常用的反应容器），确定传质限制是这种降低的一个重要决定因素。两种与传质效率相关的因素——混合速度和瓶填充——在固体负载高于 15%DM 时，与纤维素转化率密切相关。底物特性在传质性能中的作用也很重要，这一点通过在相同固体负载下用两种不同预处理方法的玉米秸秆底物进行糖化得到了揭示。几种方法，包括预混合、分批进料操作，特别是使用水平旋转反应器，通过提高固体浓度高于 15%DM 时的传质效率，被证明可以有效地促进纤维素的转化。
