Association of Interleukin-1B gene Polymorphism with infected Dyspeptic Gastric Diseases and Healthy Population.

OBJECTIVE

The aim of study was to investigate the association of IL 1B gene polymorphism with involvement of and other gastric diseases.

METHODS

Blood samples of dyspeptic patients were collected from endoscopy department of Allied Hospital Faisalabad from January 2017 to January 2019 and were qualitatively assayed for serological detection of CagA antibodies. PCR followed by direct sequencing was performed for proinflammatory IL-1B gene polymorphism detection. Sequence analysis was performed in software SnapGene viewer for haplotypes.

RESULTS

Demographic characteristics of seropositive patients showed maximum 25% gastritis in age groups of 20-40 years and 41-60 years, predominantly (41.7%) in females. While in seronegative patient's gastritis (33.3%) was found in age group of 20-40 years mainly in males (41.7%). Among studied groups, higher expression of IL-1B-511 genotype (33.3%) polymorphism was found in healthy individuals as compared to seropositive (25%) and seronegative (8.3%). While IL-1B-31 genotype showed maximum 33.3% polymorphism rate in seropositive gastric diseased group. Moreover, haplotypes frequencies IL-1B-511CC and IL-1B-31TT were predominantly (20%) found in seropositive gastric diseased group.

CONCLUSIONS

In seropositive patients, gastric disease was commonly found, however, gastric disease was not only associated with as seronegative patients were also carrying gastric complications. Interleukin IL-1B polymorphism was partially associated with infection in studied dyspeptic population.