Saarani Nur Najiha, Jamuna-Thevi Kalitheerta, Shahab Neelam, Hermawan Hendra, Saidin Syafiqah
Faculty of Biosciences & Medical Engineering, Universiti Teknologi Malaysia.
Advanced Materials Research Center.
Dent Mater J. 2017 May 31;36(3):260-265. doi: 10.4012/dmj.2016-177. Epub 2017 Jan 20.
A guided bone regeneration (GBR) membrane has been extensively used in the repair and regeneration of damaged periodontal tissues. One of the main challenges of GBR restoration is bacterial colonization on the membrane, constitutes to premature membrane degradation. Therefore, the purpose of this study was to investigate the antibacterial efficacy of triple-layered GBR membrane composed of poly(lactic-co-glycolic acid) (PLGA), nanoapatite (NAp) and lauric acid (LA) with two types of Gram-negative periodontal bacteria, Fusobacterium nucleatum and Porphyromonas gingivalis through a disc diffusion and bacterial count tests. The membranes exhibited a pattern of growth inhibition and killing effect against both bacteria. The increase in LA concentration tended to increase the bactericidal activities which indicated by higher diameter of inhibition zone and higher antibacterial percentage. It is shown that the incorporation of LA into the GBR membrane has retarded the growth and proliferation of Gram-negative periodontal bacteria for the treatment of periodontal disease.
引导骨组织再生（GBR）膜已广泛应用于受损牙周组织的修复和再生。GBR修复的主要挑战之一是膜上的细菌定植，这会导致膜过早降解。因此，本研究的目的是通过纸片扩散法和细菌计数试验，研究由聚乳酸-乙醇酸共聚物（PLGA）、纳米磷灰石（NAp）和月桂酸（LA）组成的三层GBR膜对两种革兰氏阴性牙周细菌——具核梭杆菌和牙龈卟啉单胞菌的抗菌效果。这些膜对两种细菌均表现出生长抑制和杀灭作用。LA浓度的增加倾向于增强杀菌活性，这表现为抑菌圈直径更大和抗菌率更高。结果表明，在GBR膜中加入LA可抑制革兰氏阴性牙周细菌的生长和增殖，从而用于治疗牙周疾病。
