Department of Chemistry, Shantou University, Guangdong, 515063, People's Republic of China. Department of Mechanical and Biomedical Engineering, City University of Hong Kong, 83 Tat Chee Ave., H.K. SAR, People's Republic of China.
Methods Appl Fluoresc. 2017 Jan 23;5(1):015001. doi: 10.1088/2050-6120/aa5387.
In this paper, a new coumarin-based fluorescent probe for hydrazine was rationally designed and successfully synthesized based on the Gabriel reaction. This novel probe enabled highly sensitive and selective detection of hydrazine. The detection limit was 43.6 nM (1.49 ppb). It displayed distinct changes in the intensity of both absorption and emission spectra upon the addition of hydrazine and remarkable color changes can be visually observed. The sensing mechanism of this probe toward hydrazine was characterized by H NMR and mass spectroscopy. Moreover, with the help of theoretical calculations, the sensing capability of this probe for hydrazine detection was described: the electron structure was modulated by a three-substituted group of coumarin once upon the addition of hydrazine. In addition, a test paper experiment indicated its great potential in environment monitoring.
本文基于 Gabriel 反应，合理设计并成功合成了一种用于检测肼的新型香豆素基荧光探针。该新型探针能够对肼进行高灵敏度和选择性检测，检测限为 43.6 nM（1.49 ppb）。探针在加入肼后，其吸收和发射光谱的强度均发生了明显变化，并且可以通过肉眼观察到明显的颜色变化。通过核磁共振氢谱和质谱对该探针检测肼的传感机制进行了表征。此外，借助理论计算，描述了该探针对肼检测的传感能力：一旦加入肼，香豆素的三取代基团就会调节其电子结构。此外，试纸实验表明其在环境监测方面具有巨大的应用潜力。
