Boost Maureen Valerie, Cheung Sin Wan, Cho Pauline
Centre for Myopia Research, School of Optometry, The Hong Kong Polytechnic University, Hung Hom, Hong Kong SAR, China.
Adv Ophthalmol Pract Res. 2022 Jan 20;1(2):100024. doi: 10.1016/j.aopr.2022.100024. eCollection 2021 Dec.
To determine organisms present in the conjunctiva of children before and after orthokeratology lens wear, using MALDI-TOF mass spectrometry.
Conjunctival samples were collected from children aged 8-12 years (inclusive) at baseline and on three occasions over the first six months of orthokeratology treatment. All lenses were disinfected using the povidone iodine-based solution every day after use. Specimens were cultured and all isolated colonies were identified using MALDI-TOF mass spectrometry. Numbers of organisms and diversity were compared over the study period and the presence of any ocular pathogens noted and participants informed, where appropriate, to enhance their compliance with lens care routine.
Organisms isolated from 76 children were generally similar to other studies employing culture methods. However, MALDI-TOF results yielded a wider range of species of and , as well as a few less frequently reported organisms. Only one culture yielded fungi. Ocular pathogens were only isolated from 9 subjects (4 before lens wear and 5 after lens wear), each on one occasion only. Diversity and numbers of organisms fell slowly over the period of the study, but the changes were not significant.
Lens wear did not affect the overall content of the ocular microbiome, but the diversity was somewhat reduced. The incidence of ocular pathogens was low, suggesting that risk of ocular infection was not substantially increased by orthokeratology treatment using a povidone-iodine disinfecting solution.
使用基质辅助激光解吸电离飞行时间质谱法（MALDI-TOF）确定角膜塑形镜佩戴前后儿童结膜中的微生物。
收集8至12岁（含）儿童在基线时以及角膜塑形镜治疗前六个月内三个时间点的结膜样本。所有镜片每天使用后均用聚维酮碘基溶液进行消毒。对样本进行培养，并使用MALDI-TOF质谱法鉴定所有分离出的菌落。在研究期间比较微生物数量和多样性，并记录任何眼部病原体的存在情况，在适当情况下告知参与者，以提高他们对镜片护理常规的依从性。
从76名儿童中分离出的微生物与其他采用培养方法的研究结果大致相似。然而，MALDI-TOF结果显示出更广泛的物种种类，以及一些较少报道的微生物。仅一次培养分离出真菌。眼部病原体仅从9名受试者中分离出（4名在佩戴镜片前，5名在佩戴镜片后），且均仅在一次检测中分离出。在研究期间，微生物的多样性和数量缓慢下降，但变化不显著。
佩戴镜片并未影响眼部微生物群的总体组成，但多样性有所降低。眼部病原体的发生率较低，这表明使用聚维酮碘消毒溶液进行角膜塑形镜治疗不会大幅增加眼部感染的风险。
