Department of Chemical Engineering, University of Salamanca, Plaza Los Caídos s/n, 37008 Salamanca, Spain.
Department of Chemical Engineering, University of Salamanca, Plaza Los Caídos s/n, 37008 Salamanca, Spain.
Carbohydr Polym. 2017 Mar 15;160:26-33. doi: 10.1016/j.carbpol.2016.12.025. Epub 2016 Dec 9.
New drug delivery systems (DDSs) with levan or its carboxymethylated form, as carriers, and 5-fluorouracil as a drug, are produced in this work. Levan is obtained after cultivating A. nectaris and polymer nanoparticles are created in water by a self-assembled process. The effect of pH and the ionic strength on polymer nanoparticles aggregation is studied. Basic pHs produces a particle size between 300 and 400nm with a Z-potential around -20mV because a basic medium promotes repulsion forces. DDSs of 300-400nm and a Z-potential about -25mV are prepared by taking advantage of the amphiphilic properties of the levan. The drug is bound to either levan or carboxymethyllevan surfaces by electrostatic interactions, obtaining the best results at basic pHs. 45-70% of the drug is released from the levan in 23h depending on the pH preparation, whereas only a low percentage of the drug is released from the carboxymethyllevan.
本文制备了以菊糖或其羧甲基化形式为载体，5-氟尿嘧啶为药物的新型药物传递系统（DDS）。菊糖通过培养 A. nectaris 获得，聚合物纳米粒子通过自组装过程在水中形成。研究了 pH 值和离子强度对聚合物纳米粒子聚集的影响。碱性条件下产生粒径在 300-400nm 之间、Zeta 电位约为-20mV 的粒子，因为碱性介质促进了斥力。利用菊糖的两亲性，制备了粒径为 300-400nm、Zeta 电位约为-25mV 的 DDS。药物通过静电相互作用结合到菊糖或羧甲基菊糖表面，在碱性 pH 值下获得最佳效果。根据制备的 pH 值，菊糖在 23 小时内释放 45-70%的药物，而羧甲基菊糖仅释放少量药物。
