Alleviation of lead-induced oxidative stress and immune damage by selenium in chicken bursa of Fabricius.

We investigated lead (Pb)-induced oxidative stress and immune damage in the chicken bursa of Fabricius (BF) and the ameliorative effect of selenium (Se). Seven-day-old male chickens were randomly divided into four groups and were provided standard diet and drinking water, NaSeO added to the standard diet and drinking water, standard diet and (CHCOO)Pb added to drinking water, and NaSeO added to the standard diet and (CHCOO)Pb added to drinking water for 30, 60, and 90 days. The presence of Pb inhibited total antioxidant capacity (T-AOC), glutathione peroxidase (GPx), glutathione S-transferase (GST), superoxide dismutase (SOD), and catalase (CAT) activities; decreased glutathione (GSH) content; increased malondialdehyde (MDA) and hydrogen peroxide (HO) contents; inhibited interleukin (IL)-2 and interferon-γ (IFN-γ) messenger RNA (mRNA) expression; and increased IL-4, IL-6, IL-10, IL-12β, and IL-17 mRNA expression. The presence of Se relieved all of the above Pb-induced changes. There were close correlations among GSH, CAT, T-AOC, SOD, GPx, MDA, and HO and among IL-2, IL-4, IL-6, IL-12β, IL-17, and IFN-γ. Our data showed that Pb caused oxidative stress and immune damage in the chicken BF. Se alleviated Pb-induced oxidative stress and immune damage in the chicken BF.