用三种不同方法评估己内酰胺和安息香对小鼠和大鼠肝脏的DNA损伤。

DNA damage in mouse and rat liver by caprolactam and benzoin, evaluated with three different methods.

作者信息

Parodi S, Abelmoschi M L, Balbi C, De Angeli M T, Pala M, Russo P, Taningher M, Santi L

机构信息

Department of Clinical and Experimental Oncology, University of Genoa, Italy.

出版信息

Mutat Res. 1989 Nov;224(3):379-84. doi: 10.1016/0165-1218(89)90185-7.

DOI:10.1016/0165-1218(89)90185-7

PMID:2811930

Abstract

Benzoin and caprolactam were examined for their capability of inducing alkaline DNA fragmentation in mouse and rat liver DNA after treatment in vivo. Three different methods were used. With the alkaline elution technique we measured an effect presumably related to the conformation of the DNA coil. With a viscometric and a fluorometric unwinding method we measured an effect presumably related to the number of unwinding points in DNA. For both compounds only the alkaline elution technique was clearly positive. The results suggest that both caprolactam and benzoin can induce an important change in the conformation of the DNA coil without inducing true breaks in DNA.

摘要

对安息香和己内酰胺在体内处理后诱导小鼠和大鼠肝脏DNA碱性片段化的能力进行了研究。使用了三种不同的方法。通过碱性洗脱技术，我们测量了一种可能与DNA螺旋构象相关的效应。通过粘度测定法和荧光解旋法，我们测量了一种可能与DNA解旋点数量相关的效应。对于这两种化合物，只有碱性洗脱技术显示出明显的阳性结果。结果表明，己内酰胺和安息香都能诱导DNA螺旋构象发生重要变化，而不会诱导DNA产生真正的断裂。