Zeng Qi, Xie Hui, Song Hongjin, Nie Fayu, Wang Jiahua, Chen Dan, Wang Fu
School of Life Science and Technology, Xidian University, Xi'an, China.
Evid Based Complement Alternat Med. 2016;2016:6568528. doi: 10.1155/2016/6568528. Epub 2016 Dec 29.
(Leguminosae sp.) is a traditionally used remedy for treating rheumatism, blood stasis, and internal injuries. In order to reveal a new insight of the utilization of the plant, solvent extraction by ethyl acetate (EA) was performed in order to evaluate the plant extracts' in vivo excision and incision-wound potentials with models. The contents of the EA fraction, wound healing activity, acute oral toxicity, and acute dermal toxicity were studied. As a result, the main chemical constituents of the EA fraction were alkaloids, flavonoids, and steroids. The acute oral toxicity test results and assessment of skin hypoallergenicity showed that the plant extract was safe at LD50 as high as 5000 mg/kg. Both excision and incision model tests results indicated that the EA fraction of showed a significant wound healing capacity at a concentration of 5% (v/w) ( < 0.01) as observed by the increased wound contraction, decreased epithelialization time, and increased hydroxyproline content compared to the ones of the controls. The present study showed that the EA fraction of possesses potential wound healing activities and provided recent results for the use of for wound curing.
（豆科植物）是一种传统上用于治疗风湿、血瘀和内伤的药物。为了揭示该植物利用的新见解，采用乙酸乙酯（EA）进行溶剂萃取，以便用模型评估植物提取物的体内切除和切口创伤潜力。研究了EA馏分的含量、伤口愈合活性、急性经口毒性和急性皮肤毒性。结果表明，EA馏分的主要化学成分是生物碱、黄酮类化合物和甾体类化合物。急性经口毒性试验结果和皮肤低过敏性评估表明，该植物提取物在高达5000 mg/kg的半数致死剂量（LD50）下是安全的。切除模型试验和切口模型试验结果均表明，与对照组相比，5%（v/w）浓度的EA馏分通过增加伤口收缩、缩短上皮化时间和增加羟脯氨酸含量，显示出显著的伤口愈合能力（P<0.01）。本研究表明，该植物的EA馏分具有潜在的伤口愈合活性，并为该植物用于伤口治疗提供了最新结果。
