Abdoulaye Assane Hamidou, Cheng Jiasen, Fu Yanping, Jiang Daohong, Xie Jiatao
State Key Laboratory of Agricultural Microbiology, Huazhong Agricultural University, Wuhan, 430070, Hubei, People's Republic of China.
Provincial Key Laboratory of Plant Pathology of Hubei Province, Plant Pathology Department, College of Plant Science and Technology, Huazhong Agricultural University, Wuhan, 430070, Hubei, People's Republic of China.
Arch Virol. 2017 May;162(5):1409-1412. doi: 10.1007/s00705-017-3229-0. Epub 2017 Jan 25.
A double-stranded RNA (dsRNA) segment was isolated from the filamentous phytopathogenic fungus Rhizoctonia oryzae-sativae and its full-length cDNA sequence (3038 nucleotides) was determined. Sequence analysis revealed that a large open reading frame (ORF) is present on the positive strand of this dsRNA segment when the mitochondrial genetic code was applied. The ORF encodes a putative RNA-dependent RNA polymerase, which shares the closest similarity with Rhizoctonia mitovirus 1 and Rhizophagus sp. RF1 mitovirus, with 43% and 29% identity, respectively. This dsRNA segment represents the replication form of a novel mitovirus that was temporarily designated Rhizoctonia oryzae-sativae mitovirus 1 (RoMV1). Phylogenetic analysis further suggested that RoMV1 belongs to the family Narnaviridae. This is the first study to report a mitovirus genome sequence in the phytopathogenic fungus R. oryzae-sativae.
从丝状植物病原真菌水稻纹枯病菌中分离出一个双链RNA（dsRNA）片段，并测定了其全长cDNA序列（3038个核苷酸）。序列分析表明，当应用线粒体遗传密码时，该dsRNA片段的正链上存在一个大的开放阅读框（ORF）。该ORF编码一种假定的RNA依赖性RNA聚合酶，与水稻纹枯病菌线粒体病毒1和根内球囊霉属RF1线粒体病毒的相似度最高，同一性分别为43%和29%。这个dsRNA片段代表了一种新型线粒体病毒的复制形式，该病毒被暂时命名为水稻纹枯病菌线粒体病毒1（RoMV1）。系统发育分析进一步表明，RoMV1属于arna病毒科。这是首次报道水稻纹枯病菌中一种线粒体病毒基因组序列的研究。
