Zhang Tao, Li Wei, Chen Huaigu, Yu Hanshou
College of life Sciences, Nanjing Agricultural University, Nanjing, 210095, Jiangsu, People's Republic of China.
Arch Virol. 2015 Jul;160(7):1815-8. doi: 10.1007/s00705-015-2431-1. Epub 2015 Apr 28.
A putative novel mitovirus was found in isolate R1084 of the fungus Rhizoctonia cerealis, the causal agent of sharp eyespot of wheat in China. The full genome sequence of the virus was determined and analyzed. The complete cDNA sequence is 3149 nucleotides long with 59.7% A+T content. Using either the fungal mitochondrial or universal genetic code, the viral genome was found to contain a single large open reading frame that is predicted to encode a protein of 812 amino acids with an RNA-dependent RNA polymerase (RdRp) domain that is conserved in the mitovirus RdRp superfamily. The amino acid sequence of the RdRp domain is only 50% identical to the corresponding domain in Sclerotinia sclerotiorum mitovirus 11, and therefore, this virus is proposed to be a novel mitovirus, designated as Rhizoctonia cerealis mitovirus 1-R1084 (RcMV1-R1084). The distinct codon usage of RcMV1-R1084 hints that this virus is potentially able to replicate not only in mitochondria but also in the cytoplasm. This is the first report of a full-length genomic sequence of a putative mitovirus in R. cerealis.
在中国，从小麦纹枯病的病原菌禾谷丝核菌的分离株R1084中发现了一种假定的新型线粒体病毒。测定并分析了该病毒的全基因组序列。完整的cDNA序列长3149个核苷酸，A+T含量为59.7%。使用真菌线粒体遗传密码或通用遗传密码，发现病毒基因组包含一个单一的大开放阅读框，预计编码一个812个氨基酸的蛋白质，该蛋白质具有在线粒体病毒RdRp超家族中保守的RNA依赖性RNA聚合酶（RdRp）结构域。RdRp结构域的氨基酸序列与核盘菌线粒体病毒11中相应结构域的同源性仅为50%，因此，该病毒被认为是一种新型线粒体病毒，命名为禾谷丝核菌线粒体病毒1-R1084（RcMV1-R1084）。RcMV1-R1084独特的密码子使用情况表明，这种病毒可能不仅能够在线粒体中复制，还能在细胞质中复制。这是关于禾谷丝核菌中一种假定线粒体病毒全长基因组序列的首次报道。
