National Institute for Basic Biology, Division of Evolutionary Biology, Okazaki 444-8585, Japan.
Department of Basic Biology, School of Life Science, SOKENDAI (The Graduate University for Advanced Studies), Okazaki 444-8585, Japan.
Nat Commun. 2017 Jan 27;8:14242. doi: 10.1038/ncomms14242.
Both land plants and metazoa have the capacity to reprogram differentiated cells to stem cells. Here we show that the moss Physcomitrella patens Cold-Shock Domain Protein 1 (PpCSP1) regulates reprogramming of differentiated leaf cells to chloronema apical stem cells and shares conserved domains with the induced pluripotent stem cell factor Lin28 in mammals. PpCSP1 accumulates in the reprogramming cells and is maintained throughout the reprogramming process and in the resultant stem cells. Expression of PpCSP1 is negatively regulated by its 3'-untranslated region (3'-UTR). Removal of the 3'-UTR stabilizes PpCSP1 transcripts, results in accumulation of PpCSP1 protein and enhances reprogramming. A quadruple deletion mutant of PpCSP1 and three closely related PpCSP genes exhibits attenuated reprogramming indicating that the PpCSP genes function redundantly in cellular reprogramming. Taken together, these data demonstrate a positive role of PpCSP1 in reprogramming, which is similar to the function of mammalian Lin28.
陆地植物和后生动物都具有将分化细胞重编程为干细胞的能力。在这里，我们表明，苔藓植物Physcomitrella patens 的冷休克结构域蛋白 1（PpCSP1）调节分化的叶细胞向颈卵器顶端茎细胞的重编程，并且与哺乳动物中的诱导多能干细胞因子 Lin28 具有保守结构域。PpCSP1 在重编程细胞中积累，并在整个重编程过程中和在产生的干细胞中维持。PpCSP1 的表达受其 3'-非翻译区（3'-UTR）的负调控。去除 3'-UTR 可稳定 PpCSP1 转录物，导致 PpCSP1 蛋白积累并增强重编程。PpCSP1 和三个密切相关的 PpCSP 基因的四重缺失突变体表现出减弱的重编程，表明 PpCSP 基因在细胞重编程中具有冗余功能。总之，这些数据表明 PpCSP1 在重编程中具有积极作用，类似于哺乳动物 Lin28 的功能。
