Department of Biophysics, Faculty of Science, Islamic Azad University-Mashhad Branch, Mashhad, Iran.
Immunology Research Center, BuAli Research Institute, Mashhad University of Medical Sciences, Mashhad, Iran.
Cancer Biomark. 2017;18(4):349-356. doi: 10.3233/CBM-160127.
Matrix metalloproteinases (MMPs) can degrade essentially the extracellular matrix (ECM) components. MMPs are important regulators of tumor growth; hence the enzymes are considered as important targets for cancer therapy. MMP-13 is specially activated in gastric cancer and promotes the invasiveness of the primary tumors. Helicobacter Pylori (H.pylori) interacts with gastric epithelial cells and stimulates it to produce MMP-13in vitro.
The relation between MMP-13 gene expression and clinicopathological characteristics of gastric cancer in the presence of H.pylori infection was investigated in fifty patients.
The level of MMP-13 gene expression was measured by quantitative Real-time PCR method and was evaluated between two groups of normal and carcinomatous tissues.
The results showed 30% elevation of MMP-13 expression in tumor tissues. H.pylori infection did not have a significant effect on the expression of MMP-13. There was a correlation between gene expression and tumor type (P value = 0.032). In addition, there was a significant correlation between MMP-13 gene expression and tumor stage in intestinal group (P value = 0.023).
Based on the results, it might be concluded that in intestinal group, immune system plays an important role in reducing gene expression. Results also showed over expression (60%) in diffuse group. These findings suggest that using MMP-13 inhibitors in diffuse group might contribute to the control of tumor growth.
基质金属蛋白酶（MMPs）可以降解细胞外基质（ECM）的基本成分。MMPs 是肿瘤生长的重要调节剂；因此，这些酶被认为是癌症治疗的重要靶点。MMP-13 专门在胃癌中被激活，并促进原发性肿瘤的侵袭性。幽门螺杆菌（H.pylori）与胃上皮细胞相互作用，并刺激其在体外产生 MMP-13。
在 H.pylori 感染的情况下，研究 MMP-13 基因表达与胃癌的临床病理特征之间的关系，共调查了 50 名患者。
通过定量实时 PCR 方法测量 MMP-13 基因表达水平，并在正常和癌组织两组之间进行评估。
结果显示肿瘤组织中 MMP-13 表达升高 30%。H.pylori 感染对 MMP-13 的表达没有显著影响。基因表达与肿瘤类型之间存在相关性（P 值=0.032）。此外，在肠型中，MMP-13 基因表达与肿瘤分期之间存在显著相关性（P 值=0.023）。
基于这些结果，可以得出结论，在肠型中，免疫系统在降低基因表达方面发挥重要作用。弥漫型中也表现出过度表达（60%）。这些发现表明，在弥漫型中使用 MMP-13 抑制剂可能有助于控制肿瘤生长。
