Zhang Yao, An Fa-Liang, Huang Shan-Shan, Yang Lei, Gu Yu-Cheng, Luo Jun, Kong Ling-Yi
State Key Laboratory of Natural Medicines, Department of Natural Medicinal Chemistry, China Pharmaceutical University, 24 Tong Jia Xiang, Nanjing, 210009, China.
Syngenta Jealott's Hill International Research Centre, Bracknell, Berkshire, RG42 6EY, UK.
Phytochemistry. 2017 Apr;136:108-118. doi: 10.1016/j.phytochem.2017.01.008. Epub 2017 Jan 24.
Four 18 (13 → 14)-abeo limonoids, five cedrelone limonoids, and five walsurin limonoids were isolated from the fruits of Walsura robusta together with 21 known compounds. Their structures were determined by extensive studies of their one- and two-dimensional NMR spectra and mass spectroscopy results. Among all the isolated compounds, the absolute configurations of 11-oxo-dihydrocedrelone, walsuronoid B, dysoxylumosin G, and walsunoid H were confirmed by single crystal X-ray diffraction. Selected compounds were evaluated for their reversal of multidrug resistance (MDR) phenotype in human breast cancer cells in vitro. Walsurin A showed significant effect in sensitization of these resistant cancer cells at non-toxic concentration to doxorubicin with the modulation factor of 62.76.
从粗壮割舌树果实中分离得到4个18(13→14)-去甲柠檬苦素、5个杜楝酮柠檬苦素和5个沃苏林柠檬苦素，以及21个已知化合物。通过对其一维、二维核磁共振光谱和质谱结果的广泛研究确定了它们的结构。在所有分离得到的化合物中，11-氧代-二氢杜楝酮、沃苏林类化合物B、木果楝素G和沃苏林类化合物H的绝对构型通过单晶X射线衍射得以确证。对所选化合物在体外人乳腺癌细胞中逆转多药耐药（MDR）表型的能力进行了评估。沃苏林A在无毒浓度下对这些耐药癌细胞具有显著的增敏作用，对阿霉素的调节因子为62.76。
