一种与人类癌细胞相关的核蛋白优先结合于一段人类重复DNA序列。

Law M L, Gao J Z, Puck T T

Sam and Freda Davis Laboratory, Eleanor Roosevelt Institute for Cancer Research, Denver, CO 80206.

Proc Natl Acad Sci U S A. 1989 Nov;86(21):8472-6. doi: 10.1073/pnas.86.21.8472.

A protein (Rp66) of 66 kDa was shown by DNA-binding protein blot assay to bind to a human repetitive DNA sequence (low-repeat sequence; LRS) in each of 10 transformed human cell lines examined. This protein-DNA interaction was not observed in 11 normal human cell cultures or in the Chinese hamster cell line CHO-K1. Gel retardation assay confirmed the specificity of the protein-DNA binding between Rp66 and LRS. In a histiocytic lymphoma human cell line, U937, that can be induced to differentiate in the presence of phorbol ester, this binding disappeared after cell differentiation. These together with other results cited suggest a regulatory role for these repetitive sequences in the human genome, with particular application to cancer.

通过DNA结合蛋白印迹分析表明，在所检测的10种转化人细胞系中，一种66kDa的蛋白质（Rp66）可与一种人类重复DNA序列（低重复序列；LRS）结合。在11种正常人细胞培养物或中国仓鼠细胞系CHO-K1中未观察到这种蛋白质与DNA的相互作用。凝胶阻滞分析证实了Rp66与LRS之间蛋白质与DNA结合的特异性。在一种组织细胞淋巴瘤人细胞系U937中，该细胞在佛波酯存在下可被诱导分化，细胞分化后这种结合消失。这些结果以及引用的其他结果表明，这些重复序列在人类基因组中具有调节作用，尤其适用于癌症。