Co-existence of Ras activation in a chemotactic signal transduction pathway and in an autonomous wave - forming system.

The activation of Ras is common to two activities in cells of : the directed movement in a gradient of chemoattractant and the autonomous generation of propagating waves of actin polymerization on the substrate-attached cell surface. We produced large cells by electric-pulse induced fusion to simultaneously study both activities in one cell. For imaging, a fluorescent label for activated Ras was combined with labels for filamentous actin, PIP3, or PTEN. Chemotactic responses were elicited in a diffusion gradient of cyclic AMP. Waves initiated at sites separate from the front of the cell propagated in all directions. Nevertheless, the wave-forming cells were capable of recognizing the attractant gradient and managed to migrate in its direction.