Bjartell A, Persson P, Absood A, Sundler F, Håkanson R
Department of Medical Cell Research, University of Lund, Sweden.
Regul Pept. 1989 Aug;26(1):27-34. doi: 10.1016/0167-0115(89)90101-8.
Helodermin, a VIP/secretin-like peptide, was first isolated from the venom of the lizard Gila monster. Small amounts of helodermin-like peptides have since been detected in many mammalian tissues. Notably high concentrations were demonstrated in the thyroid gland, and immunocytochemical studies revealed intense helodermin-like immunostaining in thyroid C cells and medullary thyroid carcinoma cells. In the present study, we examined the adrenal gland of mouse, rat and pig for the presence of helodermin-like peptides. Using an antiserum raised against lizard helodermin immunostaining was observed in the noradrenaline-producing cells of the adrenal medulla in all 3 species. Radioimmunoassay revealed high concentrations of helodermin-like peptides in the mouse and rat adrenal. The concentrations in the pig adrenal could not be determined because of a non-parallel dilution curve. Upon high-performance liquid chromatography, the immunoreactive material in extracts of mouse and rat adrenals eluted in one major peak, close to the elution position of lizard helodermin.
蛙皮素，一种与血管活性肠肽/促胰液素类似的肽，最初是从大毒蜥的毒液中分离出来的。此后，在许多哺乳动物组织中都检测到了少量的类蛙皮素肽。值得注意的是，在甲状腺中发现了高浓度的此类肽，免疫细胞化学研究显示甲状腺C细胞和甲状腺髓样癌细胞中有强烈的类蛙皮素免疫染色。在本研究中，我们检查了小鼠、大鼠和猪的肾上腺中是否存在类蛙皮素肽。使用针对蜥蜴蛙皮素产生的抗血清，在所有这3个物种的肾上腺髓质中产生去甲肾上腺素的细胞中均观察到免疫染色。放射免疫分析显示，小鼠和大鼠肾上腺中类蛙皮素肽的浓度很高。由于稀释曲线不平行，无法确定猪肾上腺中的浓度。在高效液相色谱分析中，小鼠和大鼠肾上腺提取物中的免疫反应性物质在一个主要峰中洗脱，接近蜥蜴蛙皮素的洗脱位置。
