Politecnico di Milano, Dipartimento di Fisica, Milano, Italy.
Istituto Italiano di Tecnologia, Center for Nano Science and Technology at Polimi, Milano, Italy.
J Biomed Opt. 2017 Jan 1;22(1):15006. doi: 10.1117/1.JBO.22.1.015006.
Reduction in scattering, high absorption, and spectral features of tissue constituents above 1000 nm could help in gaining higher spatial resolution, penetration depth, and specificity for in vivo studies, opening possibilities of near-infrared diffuse optics in tissue diagnosis. We present the characterization of collagen absorption over a broadband range (500 to 1700 nm) and compare it with spectra presented in the literature. Measurements were performed using a time-domain diffuse optical technique. The spectrum was extracted by carefully accounting for various spectral distortion effects, due to sample and system properties. The contribution of several tissue constituents (water, lipid, collagen, oxy, and deoxy-hemoglobin) to the absorption properties of a collagen-rich in vivo bone location, such as radius distal in the 500- to 1700-nm wavelength region, is also discussed, suggesting bone diagnostics as a potential area of interest.
减少散射、高吸收以及组织成分在 1000nm 以上的光谱特征有助于提高体内研究的空间分辨率、穿透深度和特异性，为近红外漫射光学在组织诊断中的应用开辟了可能性。我们展示了在宽带范围内（500nm 至 1700nm）对胶原吸收的特性进行了表征，并与文献中呈现的光谱进行了比较。测量使用时域漫射光学技术进行。通过仔细考虑由于样品和系统特性引起的各种光谱失真效应，提取了光谱。还讨论了几种组织成分（水、脂质、胶原、氧合和脱氧血红蛋白）对富含胶原的体内骨位置（如半径远端在 500nm 至 1700nm 波长范围内）吸收特性的贡献，提示骨诊断可能是一个感兴趣的领域。
