Key Laboratory of Pollinating Insect Biology , Institute of Apicultural Research, Chinese Academy of Agricultural Sciences , Beijing 100093 , China.
Beijing University of Agriculture , Beijing 102206 , China.
J Agric Food Chem. 2018 Jul 25;66(29):7786-7793. doi: 10.1021/acs.jafc.8b02212. Epub 2018 Jul 11.
Effects of glyphosate on survival, developmental rate, larval weight, and midgut bacterial diversity of Apis mellifera were tested in the laboratory. Larvae were reared in vitro and fed diet containing glyphosate 0.8, 4, and 20 mg/L. The dependent variables were compared with negative control and positive control (dimethoate 45 mg/L). Brood survival decreased in 4 or 20 mg/L glyphosate treatments but not in 0.8 mg/L, and larval weight decreased in 0.8 or 4 mg/L glyphosate treatments. Exposure to three concentrations did not affect the developmental rate. Furthermore, the intestinal bacterial communities were determined using high-throughput sequencing targeting the V3-V4 regions of the 16S rDNA. All core honey bee intestinal bacterial phyla such as Proteobacteria (30.86%), Firmicutes (13.82%), and Actinobacteria (11.88%) were detected, and significant changes were found in the species diversity and richness in 20 mg/L glyphosate group. Our results suggest that high concentrations of glyphosate are deleterious to immature bees.
在实验室中测试了草甘膦对蜜蜂生存、发育速度、幼虫体重和中肠细菌多样性的影响。幼虫在体外饲养，并喂食含有草甘膦 0.8、4 和 20 mg/L 的饮食。将依赖变量与阴性对照和阳性对照（乐果 45 mg/L）进行比较。在 4 或 20 mg/L 草甘膦处理中，幼虫存活率下降，但在 0.8 mg/L 中未下降，在 0.8 或 4 mg/L 草甘膦处理中，幼虫体重下降。暴露于三种浓度均未影响发育速度。此外，使用靶向 16S rDNA V3-V4 区的高通量测序来确定肠道细菌群落。所有核心蜜蜂肠道细菌门，如变形菌门（30.86%）、厚壁菌门（13.82%）和放线菌门（11.88%）均被检测到，并且在 20 mg/L 草甘膦组中发现了物种多样性和丰富度的显著变化。我们的结果表明，高浓度的草甘膦对不成熟的蜜蜂有害。
