Pepoyan A Z, Balayan M H, Manvelyan A M, Mamikonyan V, Isajanyan M, Tsaturyan V V, Kamiya S, Netrebov V, Chikindas M L
Food Safety and Biotechnology Department, Armenian National Agrarian University, Yerevan, Armenia.
International Association for Human and Animals Health Improvement, Yerevan, Armenia.
Lett Appl Microbiol. 2017 Apr;64(4):254-260. doi: 10.1111/lam.12722. Epub 2017 Feb 27.
Previously, we reported a positive effect the probiotic formulation, Lactobacillus acidophilus INMIA 9602 Er-2 strain 317/402 (Narine strain), had on the blood characteristics of patients with familial Mediterranean fever disease (FMF). The aim of this investigation was to evaluate the effect of the Narine probiotic on growth characteristics in the predominant commensal Escherichia coli isolates from the gut microbiota in FMF-positive study participants. Bacterial growth of 192 prevalent commensal E. coli isolates found in the volunteer participants' guts was evaluated using Verhulst's logistic function. This study showed that the duration of the preparatory growth phase for the E. coli isolates collected from FMF-positive volunteers was significantly shorter, whereas the duration of the logarithmic growth phase was significantly longer (P < 0·03) than that of the isolates collected from healthy participants. The Narine probiotic formulation caused a significant extension (P < 0·001) of the preparatory growth phase in the commensal E. coli isolated from FMF subjects a month after the Narine probiotic administration was terminated. The data suggest that the mathematical model characterizes the growth of commensal E. coli isolates from FMF-positive participants and it can be useful in a decision-making process on the practical use of probiotics during FMF.
This is the first study to demonstrate the effects of Narine, containing the probiotic Lactobacillus acidophilus, on the growth of gut commensal Escherichia coli from study participants with familial Mediterranean fever disease (FMF). Verhulst's logistic function was demonstrated to act as a possible tool for the evaluation and quantification of effects produced by the probiotic formulation in FMF participants.
此前，我们报道了益生菌制剂嗜酸乳杆菌INMIA 9602 Er - 2菌株317/402（纳林菌株）对家族性地中海热疾病（FMF）患者血液特征的积极影响。本研究的目的是评估纳林益生菌对FMF阳性研究参与者肠道微生物群中主要共生大肠杆菌分离株生长特征的影响。使用韦尔哈斯特逻辑函数评估了在志愿者肠道中发现的192株常见共生大肠杆菌分离株的细菌生长情况。本研究表明，从FMF阳性志愿者收集的大肠杆菌分离株的准备生长期明显更短，而对数生长期明显更长（P < 0·03），比从健康参与者收集的分离株更长。在纳林益生菌给药终止一个月后，纳林益生菌制剂使从FMF受试者分离的共生大肠杆菌的准备生长期显著延长（P < 0·001）。数据表明，该数学模型表征了FMF阳性参与者共生大肠杆菌分离株的生长情况，并且在FMF期间益生菌实际使用的决策过程中可能有用。
这是第一项证明含有益生菌嗜酸乳杆菌的纳林对家族性地中海热疾病（FMF）研究参与者肠道共生大肠杆菌生长影响的研究。韦尔哈斯特逻辑函数被证明是评估和量化FMF参与者中益生菌制剂产生的影响的一种可能工具。
