Mirzabekyan Susanna, Harutyunyan Natalya, Manvelyan Anahit, Malkhasyan Lilit, Balayan Marine, Miralimova Shakhlo, Chikindas Michael L, Chistyakov Vladimir, Pepoyan Astghik
Division of Food Safety and Biotechnology, Armenian National Agrarian University, Yerevan 0009, Armenia.
Institute of Microbiology, Academy of Sciences of the Republic of Uzbekistan, Tashkent 100125, Uzbekistan.
Microorganisms. 2023 Feb 27;11(3):595. doi: 10.3390/microorganisms11030595.
The properties of intestinal bacteria/probiotics, such as cell surface hydrophobicity (CSH), auto-aggregation, and biofilm formation ability, play an important role in shaping the relationship between the bacteria and the host. The current study aimed to investigate the cell surface properties of fish intestinal bacteria and probiotics. Microbial adhesion to hydrocarbons was tested according to Kos and coauthors. The aggregation abilities of the investigated strains were studied as described by Collado and coauthors. The ability of bacterial isolates to form a biofilm was determined by performing a qualitative analysis using crystal violet staining based on the attachment of bacteria to polystyrene. These studies prove that bacterial cell surface hydrophobicity (CSH) is associated with the growth medium, and the effect of the growth medium on CSH is species-specific and likely also strain-specific. Isolates of intestinal lactobacilli from fish () differed from isolates of non-fish/shrimp origin in the relationship between auto-aggregation and biofilm formation. Average CSH levels for fish lactobacilli and might were lower compared to those of non-fish origin, which may affect the efficiency of non-fish probiotics use in fisheries due to the peculiarities of the hosts' aquatic lifestyles.
肠道细菌/益生菌的特性，如细胞表面疏水性（CSH）、自聚集和生物膜形成能力，在塑造细菌与宿主之间的关系中起着重要作用。当前研究旨在调查鱼类肠道细菌和益生菌的细胞表面特性。根据科斯及其合著者的方法测试微生物对碳氢化合物的粘附性。按照科利亚多及其合著者所述研究受试菌株的聚集能力。通过基于细菌在聚苯乙烯上的附着情况进行结晶紫染色定性分析来确定细菌分离株形成生物膜的能力。这些研究证明细菌细胞表面疏水性（CSH）与生长培养基相关，且生长培养基对CSH的影响具有物种特异性，可能还具有菌株特异性。鱼类肠道乳酸杆菌分离株（）在自聚集与生物膜形成的关系上与非鱼类/虾类来源的分离株不同。与非鱼类来源的相比，鱼类乳酸杆菌和可能的平均CSH水平较低，由于宿主水生生活方式的特殊性，这可能会影响非鱼类益生菌在渔业中的使用效率。
