Ai-Lati Aisikaer, Liu Shuangping, Ji Zhongwei, Zhang Hao, Mao Jian
a National Engineering Laboratory for Cereal Fermentation Technology , Jiangnan University , Wuxi , China.
b School of Food Science and Technology , Jiangnan University , Wuxi, Jiangsu , China.
Bioengineered. 2017 Sep 3;8(5):565-571. doi: 10.1080/21655979.2017.1283459. Epub 2017 Jan 31.
In this study, a Ganoderma lucidum polysaccharide GLP-1-1 was isolated from a culture broth with Mw of 22014 Da. Monosaccharide contained glucose, mannose, and galactose with mole percentages of 92.33%, 7.55%, and 0.22%, respectively. Moreover, FTIR and methylation analysis were conducted to characterize the structural properties of GLP-1-1. The results of antioxidant activity analysis showed that GLP-1-1 had a great DPPH and ABTS radical scavenging activity. Meanwhile, GLP-1-1 also exhibited anti-tumor activity to A431 and MDA-MB-231 cells, and inhibitory rates were dose-dependent. During culturing with GLP-1-1, the G1/G0 cell percentage of A431 cells was increased from 48.64% to 84.52%, and the G1/G0 cell percentage of MDA-MB-231 cells was increased from 57.14% to 73.48%. Therefore, the anti-tumor activity of GLP-1-1 may be caused by inducing the G1/G0 arrest of tumor cells.
在本研究中，从一种分子量为22014 Da的培养液中分离出灵芝多糖GLP-1-1。单糖包含葡萄糖、甘露糖和半乳糖，摩尔百分比分别为92.33%、7.55%和0.22%。此外，进行了傅里叶变换红外光谱（FTIR）和甲基化分析以表征GLP-1-1的结构特性。抗氧化活性分析结果表明，GLP-1-1具有很强的清除DPPH和ABTS自由基的活性。同时，GLP-1-1对A431和MDA-MB-231细胞也表现出抗肿瘤活性，且抑制率呈剂量依赖性。在用GLP-1-1培养期间，A431细胞的G1/G0期细胞百分比从48.64%增加到84.52%，MDA-MB-231细胞的G1/G0期细胞百分比从57.14%增加到73.48%。因此，GLP-1-1的抗肿瘤活性可能是由诱导肿瘤细胞G1/G0期阻滞引起的。
