Zhou Huabin, Liu Gaoqiang, Huang Furu, Wu Xiangting, Yang Hailong
School of Life & Environmental Sciences, Wenzhou University, Wenzhou, 325035, China.
Arch Pharm Res. 2014 Dec;37(12):1530-7. doi: 10.1007/s12272-014-0391-8. Epub 2014 Apr 16.
Polysaccharides from Ganoderma lucidum showed multiple biological activities, such as immuno-modulating, antitumor, antioxidant, and hepatoprotective activity, etc. Adlay oil was added into the media to enhance polysaccharide production by submerged culture of G. lucidum in this work. The results revealed the optimal concentration of adlay oil was 1.5 % for polysaccharide production of G. lucidum. Analysis of the polysaccharide components confirmed that no novel components were biosynthesized by the addition of adlay oil. The main fraction of extracellular polysaccharide, GLEP-2, was isolated from the submerged culture broth of G. lucidum by ethanol precipitation, filtration, DEAE cellulose-52 and Sepharose CL-6B chromatography. GLEP-2, which was composed of glucose, galactose, mannose, arabinose, and rhamnose in a ratio of 332:55:32:13:3 respectively, had an average molecular weight of ~2.08 × 10(4) Da. The bioactivity tests demonstrated that GLEP-2 enhanced the T lymphocyte proliferation significantly at a concentration of 200 μg/mL and B lymphocyte proliferation at lower concentrations of 50 μg/mL. The results suggest polysaccharides from the submerged culture of G. lucidum are potential candidates for further development and possible commercial applications, especially in the pharmaceutical and functional foods industries.
灵芝多糖具有多种生物活性，如免疫调节、抗肿瘤、抗氧化和保肝活性等。在本研究中，将薏仁油添加到培养基中，以通过灵芝的深层培养提高多糖产量。结果表明，薏仁油对灵芝多糖产量的最佳浓度为1.5%。多糖成分分析证实，添加薏仁油并未生物合成新的成分。通过乙醇沉淀、过滤、DEAE纤维素-52和琼脂糖CL-6B柱色谱法，从灵芝深层培养液中分离出胞外多糖的主要组分GLEP-2。GLEP-2由葡萄糖、半乳糖、甘露糖、阿拉伯糖和鼠李糖按332:55:32:13:3的比例组成，平均分子量约为2.08×10(4)Da。生物活性测试表明，GLEP-2在浓度为200μg/mL时显著增强T淋巴细胞增殖，在较低浓度50μg/mL时增强B淋巴细胞增殖。结果表明，灵芝深层培养产生的多糖是进一步开发和可能商业应用的潜在候选物，特别是在制药和功能性食品工业中。
