Dumaru Rabina, Baral Ratna, Shrestha Lok Bahadur
Microbiology & Infectious Diseases, B.P Koirala Institute of Health Sciences, Dharan, 56700, Nepal.
BMC Res Notes. 2019 Jan 18;12(1):38. doi: 10.1186/s13104-019-4084-8.
Gram-negative bacilli are the common causative agents for community-acquired, nosocomial and opportunistic infections. The recent upsurge of biofilm, as well as beta-lactamases producing strains, have synergistically led to the extensive dissemination of multi-drug resistant gram-negative bacilli. This study was carried out with an intention to detect the biofilm formation by gram-negative bacilli and determine their antibiogram along with the detection of extended-spectrum beta-lactamases (ESBLs) and metallo-beta-lactamases (MBLs) production.
Among 314 isolates, Escherichia coli (38%) were the predominant isolates followed by Acinetobacter spp. (20%), Klebsiella spp. (16%), and Pseudomonas spp. (12%). Overall, 197 (62.73%) of isolates were biofilm positive. 84 (26.75%) and 51 (16.24%) were confirmed as ESBL and MBL producers respectively. The association between MBL production and biofilm formation was statistically significant (χ = 10.20, P value= 0.002) whereas it was insignificant between ESBL and biofilm production (χ = 0.006, P-value= 0.937). Most of the biofilm and MBL producing strains were multi-drug resistant.
革兰氏阴性杆菌是社区获得性感染、医院感染和机会性感染的常见病原体。近期生物膜以及产β-内酰胺酶菌株的激增，协同导致了多重耐药革兰氏阴性杆菌的广泛传播。本研究旨在检测革兰氏阴性杆菌生物膜的形成情况，确定其抗菌谱，并检测超广谱β-内酰胺酶（ESBLs）和金属β-内酰胺酶（MBLs）的产生情况。
在314株分离菌株中，大肠埃希菌（38%）是主要分离菌株，其次是不动杆菌属（20%）、克雷伯菌属（16%）和假单胞菌属（12%）。总体而言，197株（62.73%）分离菌株生物膜呈阳性。分别有84株（26.75%）和51株（16.24%）被确认为产ESBL和产MBL菌株。MBL产生与生物膜形成之间的关联具有统计学意义（χ=10.20，P值=0.002），而ESBL与生物膜产生之间的关联无统计学意义（χ=0.006，P值=0.937）。大多数产生物膜和产MBL菌株具有多重耐药性。
