Agarwal Ashish, Hegde Anupama, Yadav Charu, Ahmad Afzal, Manjrekar Poornima Ajay, Srikantiah Rukmini Mysore
Department of Biochemistry, Center for Basic Sciences, Kasturba Medical College (Manipal University), Mangalore, Karnataka 575004 India.
Indian J Clin Biochem. 2017 Mar;32(1):33-38. doi: 10.1007/s12291-016-0567-8. Epub 2016 May 3.
Impaired fasting glucose (IFG) is a high risk subclinical condition for the progression of type 2 diabetes mellitus and the hyperglycemia seen in this condition is because of the development of insulin resistance (IR). Obesity, inflammation, oxidative stress and many other factors have been implicated in development of IR in type 2 diabetes mellitus and its successive complications. Current study was aimed to ascertain the correlation of inflammation and oxidative stress markers [interleukin-6 (IL-6) and myeloperoxidase (MPO)] with IR in subjects with IFG. In this study, 80 subjects (40 IFG, 40 healthy controls) aged 25-45 years were selected based on their fasting plasma glucose (FPG) values and clinical history. Serum insulin, IL-6 and MPO were estimated by ELISA method and IR was calculated using Homeostatic Model Assessment Index 2 (HOMA 2) calculator. Pearson's correlation coefficient and independent sample '' test were used for statistical analysis. IL-6 and MPO were found to be significantly elevated in IFG group and both correlates significantly with IR (r 0.413, r 0.645). Only MPO had significant correlation with FPG (r 0.388). In conclusion, the association of altered levels of IL-6 and MPO with IR are suggestive of a role of inflammation and oxidative stress in the initiation and progression of IR in individuals with IFG.
空腹血糖受损（IFG）是2型糖尿病进展的高风险亚临床状态，这种状态下出现的高血糖是由于胰岛素抵抗（IR）的发展。肥胖、炎症、氧化应激和许多其他因素与2型糖尿病及其后续并发症中IR的发展有关。当前研究旨在确定炎症和氧化应激标志物[白细胞介素-6（IL-6）和髓过氧化物酶（MPO）]与IFG患者IR之间的相关性。在本研究中，根据空腹血糖（FPG）值和临床病史，选择了80名年龄在25至45岁之间的受试者（40名IFG患者，40名健康对照）。采用酶联免疫吸附测定法（ELISA）测定血清胰岛素、IL-6和MPO，并使用稳态模型评估指数2（HOMA 2）计算器计算IR。采用Pearson相关系数和独立样本t检验进行统计分析。发现IFG组中IL-6和MPO显著升高，且二者均与IR显著相关（r = 0.413，r = 0.645）。只有MPO与FPG有显著相关性（r = 0.388）。总之，IL-6和MPO水平改变与IR的关联表明炎症和氧化应激在IFG个体IR的发生和发展中起作用。
