• 文献检索
  • 文档翻译
  • 深度研究
  • 学术资讯
  • Suppr Zotero 插件Zotero 插件
  • 邀请有礼
  • 套餐&价格
  • 历史记录
应用&插件
Suppr Zotero 插件Zotero 插件浏览器插件Mac 客户端Windows 客户端微信小程序
定价
高级版会员购买积分包购买API积分包
服务
文献检索文档翻译深度研究API 文档MCP 服务
关于我们
关于 Suppr公司介绍联系我们用户协议隐私条款
关注我们

Suppr 超能文献

核心技术专利：CN118964589B侵权必究
粤ICP备2023148730 号-1Suppr @ 2026

文献检索

告别复杂PubMed语法，用中文像聊天一样搜索，搜遍4000万医学文献。AI智能推荐，让科研检索更轻松。

立即免费搜索

文件翻译

保留排版，准确专业，支持PDF/Word/PPT等文件格式，支持 12+语言互译。

免费翻译文档

深度研究

AI帮你快速写综述，25分钟生成高质量综述，智能提取关键信息，辅助科研写作。

立即免费体验

甲磺酸乙酯诱变的L.m M代生理变异分析

The Analysis of Physiological Variations in M Generation of L. Mutagenized by Ethyl Methane Sulfonate.

作者信息

Xi-Ou Xiao, Wenqiu Lin, Wei Li, Xiaoming Gao, Lingling Lv, Feiyue Ma, Yuge Liu

机构信息

South Subtropical Crops Research Institute, Chinese Academy of Tropical Agricultural Sciences (CATAS)Zhanjiang, China; Zhanjiang City Key Laboratory for Tropical Crops Genetic ImprovementZhanjiang, China.

出版信息

Front Plant Sci. 2017 Jan 19;8:17. doi: 10.3389/fpls.2017.00017. eCollection 2017.

DOI:10.3389/fpls.2017.00017
PMID:28154575
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC5243811/
Abstract

The eggplant was mutagenized with ethyl methane sulfonate (EMS) to enhance its genetic variability in our previous paper. In this article, we further analyzed the phenotype of M generation of mutant eggplants. A total of 325 independent M families were investigated for phenotypic variation. In addition to the visible phenotypic variation, chlorogenic acid (CGA) concentrations were analyzed in 26 fruits of mutants with High Performance Liquid Chromatography assay. Seventeen fruits exhibited significantly higher concentrations of CGAs than those in wild-type. The anthocyanin concentration of S9-1, the purple black mutant, was higher than WT, meanwhile, the anthocyanin concentration of L6-4 and U36-1 was lower than WT. Furthermore, our RT-PCR result demonstrated that the expression levels of anthocyanin biosynthetic genes, except for SmPAL, were increased in S9-1, and the regulator was decreased in L6-4 and U36-1 mutants. Together, our data indicated that, M generation showed abundant phenotypic variations and the strong potential usage for next step of breeding and molecular genetic mechanisms in eggplant.

摘要

在我们之前的论文中，用甲基磺酸乙酯（EMS）对茄子进行诱变处理以增加其遗传变异性。在本文中，我们进一步分析了突变茄子M代的表型。共对325个独立的M家系进行了表型变异调查。除了可见的表型变异外，还采用高效液相色谱法分析了26个突变体果实中的绿原酸（CGA）浓度。17个果实中CGA的浓度显著高于野生型。紫黑色突变体S9-1的花青素浓度高于野生型，而L6-4和U36-1的花青素浓度低于野生型。此外，我们的RT-PCR结果表明，除SmPAL外，S9-1中花青素生物合成基因的表达水平升高，而在L6-4和U36-1突变体中调控因子的表达水平降低。总之，我们的数据表明，M代表现出丰富的表型变异，在茄子下一步育种和分子遗传机制研究中具有很强的潜在应用价值。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/1ee9/5243811/5e498df68e84/fpls-08-00017-g009.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/1ee9/5243811/2b829eebfb56/fpls-08-00017-g001.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/1ee9/5243811/06d838b39194/fpls-08-00017-g002.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/1ee9/5243811/a973ca85b705/fpls-08-00017-g003.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/1ee9/5243811/7d43c2f7adc4/fpls-08-00017-g004.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/1ee9/5243811/201f951a9082/fpls-08-00017-g005.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/1ee9/5243811/99d7fa477df5/fpls-08-00017-g006.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/1ee9/5243811/db190369bcd6/fpls-08-00017-g007.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/1ee9/5243811/f6af7e71e8bb/fpls-08-00017-g008.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/1ee9/5243811/5e498df68e84/fpls-08-00017-g009.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/1ee9/5243811/2b829eebfb56/fpls-08-00017-g001.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/1ee9/5243811/06d838b39194/fpls-08-00017-g002.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/1ee9/5243811/a973ca85b705/fpls-08-00017-g003.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/1ee9/5243811/7d43c2f7adc4/fpls-08-00017-g004.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/1ee9/5243811/201f951a9082/fpls-08-00017-g005.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/1ee9/5243811/99d7fa477df5/fpls-08-00017-g006.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/1ee9/5243811/db190369bcd6/fpls-08-00017-g007.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/1ee9/5243811/f6af7e71e8bb/fpls-08-00017-g008.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/1ee9/5243811/5e498df68e84/fpls-08-00017-g009.jpg

相似文献

1
The Analysis of Physiological Variations in M Generation of L. Mutagenized by Ethyl Methane Sulfonate.甲磺酸乙酯诱变的L.m M代生理变异分析
Front Plant Sci. 2017 Jan 19;8:17. doi: 10.3389/fpls.2017.00017. eCollection 2017.
2
Phenylpropanoids Accumulation in Eggplant Fruit: Characterization of Biosynthetic Genes and Regulation by a MYB Transcription Factor.苯丙烷类化合物在茄子果实中的积累：生物合成基因的表征及MYB转录因子的调控
Front Plant Sci. 2016 Jan 28;6:1233. doi: 10.3389/fpls.2015.01233. eCollection 2015.
3
Anthocyanin accumulation and molecular analysis of anthocyanin biosynthesis-associated genes in eggplant (Solanum melongena L.).茄子（Solanum melongena L.）中花色苷积累和花色苷生物合成相关基因的分子分析。
J Agric Food Chem. 2014 Apr 2;62(13):2906-12. doi: 10.1021/jf404574c. Epub 2014 Mar 21.
4
Identification of candidate genes associated with less-photosensitive anthocyanin phenotype using an EMS mutant () in eggplant ( L.).利用茄子（Solanum melongena L.）的一个甲基磺酸乙酯（EMS）突变体鉴定与低光敏花青素表型相关的候选基因。
Front Plant Sci. 2023 Dec 11;14:1282661. doi: 10.3389/fpls.2023.1282661. eCollection 2023.
5
Transcriptome profiling of genes related to light-induced anthocyanin biosynthesis in eggplant (Solanum melongena L.) before purple color becomes evident.茄子（Solanum melongena L.）在紫色显现之前与光诱导花青素生物合成相关基因的转录组分析。
BMC Genomics. 2018 Mar 20;19(1):201. doi: 10.1186/s12864-018-4587-z.
6
Induced variations of ethyl methane sulfonate mutagenized cowpea ( L. walp) plants.甲磺酸乙酯诱变豇豆（L. walp）植株的诱导变异
Front Plant Sci. 2022 Aug 5;13:952247. doi: 10.3389/fpls.2022.952247. eCollection 2022.
7
Identification of Light-Independent Anthocyanin Biosynthesis Mutants Induced by Ethyl Methane Sulfonate in Turnip "Tsuda" (Brassica rapa).萝卜“津田芜菁”（Brassica rapa）中通过甲磺酸乙酯诱导产生的非光依赖型花青素生物合成突变体的鉴定
Int J Mol Sci. 2017 Jun 22;18(7):1288. doi: 10.3390/ijms18071288.
8
Characterization of Browning, Chlorogenic Acid Content, and Polyphenol Oxidase Activity in Different Varietal Types of Eggplant () for Improving Visual and Nutritional Quality.不同品种茄子（）的褐变、绿原酸含量及多酚氧化酶活性表征，以改善视觉和营养品质。
Plants (Basel). 2024 Apr 9;13(8):1059. doi: 10.3390/plants13081059.
9
Mapping and validation of the epistatic and genes controlling anthocyanin biosynthesis in the peel of eggplant ( L.) fruit.茄子（L.）果实果皮中花青素生物合成相关上位性基因及控制基因的定位与验证
Hortic Res. 2022 Dec 2;10(2):uhac268. doi: 10.1093/hr/uhac268. eCollection 2023 Feb.
10
Genome-Wide Analysis of Artificial Mutations Induced by Ethyl Methanesulfonate in the Eggplant ( L.).茄子（L.）中乙基甲磺酸诱导的人工突变的全基因组分析。
Genes (Basel). 2019 Aug 7;10(8):595. doi: 10.3390/genes10080595.

引用本文的文献

1
Correlation, Path Coefficient and Genetic Diversity Analysis in EMS Induced Cultivars of Ashwagandha [Withania somnifera (L.) Dunal].印度人参（Withania somnifera (L.) Dunal）EMS诱导品种的相关性、通径系数及遗传多样性分析
Biochem Genet. 2025 Aug 12. doi: 10.1007/s10528-025-11205-0.
2
Current knowledge and breeding strategies for management of aphid-transmitted viruses of pepper ( spp.) in Africa.非洲辣椒（辣椒属）蚜虫传播病毒的管理现状及育种策略
Front Plant Sci. 2024 Oct 25;15:1449889. doi: 10.3389/fpls.2024.1449889. eCollection 2024.
3
Transcriptome, Plant Hormone, and Metabolome Analysis Reveals the Mechanism of Purple Pericarp Formation in 'Zihui' Papaya ( L.).

本文引用的文献

1
TOMATOMA Update: Phenotypic and Metabolite Information in the Micro-Tom Mutant Resource.番茄突变体资源库（TOMATOMA）更新：Micro-Tom突变体资源中的表型和代谢物信息
Plant Cell Physiol. 2016 Jan;57(1):e11. doi: 10.1093/pcp/pcv194. Epub 2015 Dec 30.
2
Construction of a high-density mutant library in soybean and development of a mutant retrieval method using amplicon sequencing.大豆高密度突变体文库的构建及利用扩增子测序的突变体检索方法的开发。
BMC Genomics. 2015 Nov 26;16:1014. doi: 10.1186/s12864-015-2079-y.
3
Ectopic Expression of the Coleus R2R3 MYB-Type Proanthocyanidin Regulator Gene SsMYB3 Alters the Flower Color in Transgenic Tobacco.
转录组、植物激素和代谢组分析揭示‘紫慧’木瓜紫色果皮形成的机制。
Molecules. 2024 Mar 27;29(7):1485. doi: 10.3390/molecules29071485.
4
Current trends and insights on EMS mutagenesis application to studies on plant abiotic stress tolerance and development.EMS诱变在植物非生物胁迫耐受性及发育研究中的应用现状与见解
Front Plant Sci. 2023 Jan 4;13:1052569. doi: 10.3389/fpls.2022.1052569. eCollection 2022.
5
Development and Characterization of an Ethyl Methane Sulfonate (EMS) Induced Mutant Population in L.甲磺酸乙酯（EMS）诱导的L.突变群体的开发与特性分析
Plants (Basel). 2020 Mar 23;9(3):396. doi: 10.3390/plants9030396.
6
Grown to be Blue-Antioxidant Properties and Health Effects of Colored Vegetables. Part II: Leafy, Fruit, and Other Vegetables.有色蔬菜的生长特性、抗氧化特性及健康效应。第二部分：叶菜类、水果类及其他蔬菜。
Antioxidants (Basel). 2020 Jan 23;9(2):97. doi: 10.3390/antiox9020097.
7
Genome-Wide Analysis of Artificial Mutations Induced by Ethyl Methanesulfonate in the Eggplant ( L.).茄子（L.）中乙基甲磺酸诱导的人工突变的全基因组分析。
Genes (Basel). 2019 Aug 7;10(8):595. doi: 10.3390/genes10080595.
8
Identification of Light-Independent Anthocyanin Biosynthesis Mutants Induced by Ethyl Methane Sulfonate in Turnip "Tsuda" (Brassica rapa).萝卜“津田芜菁”（Brassica rapa）中通过甲磺酸乙酯诱导产生的非光依赖型花青素生物合成突变体的鉴定
Int J Mol Sci. 2017 Jun 22;18(7):1288. doi: 10.3390/ijms18071288.
彩叶草R2R3 MYB型原花青素调节基因SsMYB3的异位表达改变了转基因烟草的花色。
PLoS One. 2015 Oct 8;10(10):e0139392. doi: 10.1371/journal.pone.0139392. eCollection 2015.
4
Two forward genetic screens for vein density mutants in sorghum converge on a cytochrome P450 gene in the brassinosteroid pathway.对高粱叶脉密度突变体进行的两项正向遗传学筛选聚焦于油菜素内酯途径中的一个细胞色素P450基因。
Plant J. 2015 Oct;84(2):257-66. doi: 10.1111/tpj.13007.
5
Ethyl methane sulfonate induced mutations in M2 generation and physiological variations in M1 generation of peppers (Capsicum annuum L.).甲磺酸乙酯诱导辣椒（辣椒属）M2代发生突变以及M1代出现生理变异。
Front Plant Sci. 2015 Jun 4;6:399. doi: 10.3389/fpls.2015.00399. eCollection 2015.
6
An ACCUMULATION AND REPLICATION OF CHLOROPLASTS 5 gene mutation confers light green peel in cucumber.叶绿体 5 基因积累和复制突变导致黄瓜果皮呈淡绿色。
J Integr Plant Biol. 2015 Nov;57(11):936-42. doi: 10.1111/jipb.12355. Epub 2015 Jun 10.
7
MutMap accelerates breeding of a salt-tolerant rice cultivar.MutMap加速了耐盐水稻品种的培育。
Nat Biotechnol. 2015 May;33(5):445-9. doi: 10.1038/nbt.3188. Epub 2015 Mar 23.
8
Genome-wide survey of artificial mutations induced by ethyl methanesulfonate and gamma rays in tomato.甲磺酸乙酯和伽马射线诱导番茄人工突变的全基因组调查
Plant Biotechnol J. 2016 Jan;14(1):51-60. doi: 10.1111/pbi.12348. Epub 2015 Feb 16.
9
Location of chlorogenic acid biosynthesis pathway and polyphenol oxidase genes in a new interspecific anchored linkage map of eggplant.绿原酸生物合成途径及多酚氧化酶基因在茄子新种间锚定连锁图谱中的定位
BMC Plant Biol. 2014 Dec 10;14:350. doi: 10.1186/s12870-014-0350-z.
10
Reducing capacity, chlorogenic acid content and biological activity in a collection of scarlet (Solanum aethiopicum) and Gboma (S. macrocarpon) eggplants.一组 Scarlet（埃塞俄比亚茄）和 Gboma（大果茄）茄子的还原能力、绿原酸含量及生物活性
Int J Mol Sci. 2014 Sep 26;15(10):17221-41. doi: 10.3390/ijms151017221.