Mackey J K, Green M, Wold W S, Rigden P
J Natl Cancer Inst. 1979 Jan;62(1):23-6.
We investigated whether human adenovirus type 4 (Ad4) might cause cancer in humans. Cell culture and animal model studies indicated that Ad4-induced human tumors should contain Ad4 DNA sequences. Thus Ad4 DNA was labeled in vitro (sp act, approximately 10(7) 3H counts/min/microgram) and hybridized in liquid phase with DNA extracted from human tumors. No viral sequences were detected in 31 squamous cell carcinomas of the lung, 5 adenocarcinomas of the lung, 4 oat cell carcinomas of the lung, 4 carcinomas of the stomach, 10 carcinomas of the colon, 3 carcinomas of the kidney, 3 carcinomas of the breast, 2 carcinomas of the ovary, and 6 Hodgkin's lymphomas. Reconstruction experiments with added Ad4 DNA indicated that the probe detected about 1 copy of 5% of the Ad4 genome per tumor cell. Therefore, these data were strong evidence (but did not prove) that none of these particular tumors were induced by Ad4.
我们研究了4型人类腺病毒（Ad4）是否可能导致人类患癌。细胞培养和动物模型研究表明，Ad4诱导的人类肿瘤应含有Ad4 DNA序列。因此，Ad4 DNA在体外进行标记（比活度，约10(7) 3H计数/分钟/微克），并与从人类肿瘤中提取的DNA在液相中杂交。在31例肺鳞状细胞癌、5例肺腺癌、4例肺燕麦细胞癌、4例胃癌、10例结肠癌、3例肾癌、3例乳腺癌、2例卵巢癌和6例霍奇金淋巴瘤中均未检测到病毒序列。添加Ad4 DNA的重建实验表明，该探针在每个肿瘤细胞中可检测到约5%的Ad4基因组中的1个拷贝。因此，这些数据是有力证据（但未证实），表明这些特定肿瘤均不是由Ad4诱导的。
