Biaoxue Rong, Hua Liu, Tian Fu, Wenlong Gao
Department of Respiratory Medicine, First Affiliated Hospital, Xi'an Medical University, Xi'an.
Department of Respiratory Medicine, Gansu Provincial Hospital, Lanzhou.
Jpn J Clin Oncol. 2017 Apr 1;47(4):342-349. doi: 10.1093/jjco/hyx005.
Stathmin has been found to be involved in malignant tumors; the aim of this study was to investigate the relationship between serum stathmin and clinico-pathological features of lung cancer.
In three lung cancer cell lines, stathmin expression and its secretion level in the supernatant were examined by Western blot and enzyme-linked immunosorbent assay (ELISA). Expression of stathmin was examined by immunohistochemistry in 48 lung cancer tissues, and serum stathmin expression level was examined by ELISA in 96 patients with lung cancer and 82 normal individuals. Sensitivity and specificity of serum stathmin were determined by receiver operator characteristic curve (ROC).
In the three cell lines and their supernatant, stathmin levels were higher than those in 16 HBE cell line. Lung cancer tissues expressed higher level stathmin more frequently than normal lung tissue (P < 0.05). Serum stathmin level was significantly higher in the patients with lung cancer than in normal individuals (P < 0.001). Increased stathmin level in cancer tissue and serum were significantly associated with adenocarcinoma histology, lymph node metastasis and advanced stage. The threshold level of serum stathmin for distinguishing lung cancer from normal individuals was 1.86 ng/ml. The sensitivity and specificity were 93.7% and 91.5%, respectively.
Measurement of serum stathmin level could be a potential biomarker for lung cancer, especically those of adenocarcinoma, with lymph node metastasis and at advanced clinical stages.
已发现Stathmin与恶性肿瘤有关；本研究旨在探讨血清Stathmin与肺癌临床病理特征之间的关系。
采用蛋白质免疫印迹法和酶联免疫吸附测定（ELISA）检测三种肺癌细胞系中Stathmin的表达及其在上清液中的分泌水平。采用免疫组织化学法检测48例肺癌组织中Stathmin的表达，采用ELISA法检测96例肺癌患者和82例正常个体血清中Stathmin的表达水平。通过受试者工作特征曲线（ROC）确定血清Stathmin的敏感性和特异性。
在三种细胞系及其上清液中，Stathmin水平高于16HBE细胞系。肺癌组织中Stathmin表达水平高于正常肺组织的频率更高（P<0.05）。肺癌患者血清Stathmin水平显著高于正常个体（P<0.001）。癌组织和血清中Stathmin水平升高与腺癌组织学、淋巴结转移和晚期显著相关。区分肺癌与正常个体的血清Stathmin阈值水平为1.86 ng/ml。敏感性和特异性分别为93.7%和91.5%。
检测血清Stathmin水平可能是肺癌的潜在生物标志物，尤其是腺癌、有淋巴结转移和临床晚期的肺癌。
